Dp1187 | Dp62 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|

0Dp | 1Dp | 2Dp | 3Dp | N | 0Dp | 1Dp | 2Dp | 3Dp | N | |

1. % males (observed) | 0 | 69.0 | 29.9 | 1.0 | (197) | 0 | 61.8 | 36.2 | 2.0 | (199) |

2. % nuclei (adjusted for loss) | 3.5 | 68.4 | 27.2 | 0.9 | 100 | 3.2 | 62.2 | 32.9 | 1.7 | 100 |

3. × Dp copy number | × 0 | × 1 | × 2 | × 3 | × 0 | × 1 | × 2 | × 3 | ||

4. Dps/100 nuclei (expected) | 0 | 68.4 | 54.4 | 2.7 | 125.5 | 0 | 62.2 | 65.8 | 5.1 | 133.1 |

5. % nuclei (observed) | 23.2 | 58.0 | 17.5 | 1.3 | (69) | 61.0 | 31.4 | 7.6 | 0 | (105) |

6. × Dp copy number | × 0 | × 1 | × 2 | × 3 | × 0 | × 1 | × 2 | × 3 | ||

7. Dps/100 nuclei (observed) | 0 | 58.0 | 35.0 | 4.0 | 97 | 0 | 31.4 | 15.2 | 0 | 46.7 |

8. Univalents/100 nuclei | (Line 7 × 1/DE) | 125.5 | (Line 7 × 1/DE) | 60.4 | ||||||

9. Trivalents/100 nuclei | 0 | 0 | 0 | 0 | 0 | 0 | 34.0 | 16.3 | 0.57 | 51.0 |

10. Quadrivalents/100 nuclei | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9.8 | 1.0 | 10.8 |

All values are per 100 nuclei (or per 100 males—line 1). Line 1: Census of

*Dp1187*and*Dp62*stocks; percentages of y^{+}males carrying 0, 1, 2, or 3Dps (see materials and methods). Total males tested are in parentheses. Line 2: Expected frequencies (*ef*) of 0, 1, 2, and 3Dp spermatocytes obtained from the corresponding frequencies of males (*f*) in line 1 by adjusting for a premeiotic loss frequency of 5% for each Dp using the binomial formula. Thus*ef*(3Dp spermatocytes) =*f*(3Dp males) × (0.95)^{3}, and*ef*(2Dp spermatoctes) = (*f*(2Dp males × (0.95)^{2}) + (*f*(3Dp males) × 3 × (0.95)^{2}× 0.05)), etc. Line 3: Number of Dp copies in each genotype. Line 4: Expected numbers of Dps in spermatocytes of each class, obtained by multiplying expected spermatocyte frequencies in line 2 by Dp copy number (line 3). Line 5: Observed distribution of nuclei with 0, 1, 2, or 3 univalents. Numbers in parentheses are total numbers of nuclei scored. Line 6: Number of Dp copies in each genotype. Line 7: Observed numbers of univalents in spermatocytes of each class, obtained by multiplying univalent frequencies in line 5 by Dp copy number (line 6). Line 8: Corrected total univalents obtained by multiplying observed total univalents (line 7) by 1/DE. DE (detection efficiency) = observed/expected univalents per nucleus (*Dp1187*) = 97/125.5 = 0.773. Lines 9 and 10: Expected distributions of trivalents and quadrivalents based on expected*Dp62*frequency distribution (line 2) and*Dp62*association frequency (AF). AF = 1 − (observed/expected univalents per 100 spermatocytes) = 1 − 60.4/133.3 = 0.547. Thus trivalents/1Dp spermatocytes = 62.2 × 0.547 = 34.0; trivalents/2 Dp spermatocytes = 32.9 × 0.547 × 0.453 × 2 = 16.3; etc. In line 10, expected numbers of quadrivalents and quintivalents are combined.