TABLE 5

Results of FISH analysis of Dp1187 and Dp62 spermatocytes

Dp1187Dp62
0Dp1Dp2Dp3DpN0Dp1Dp2Dp3DpN
1. % males (observed)069.029.91.0(197)061.836.22.0(199)
2. % nuclei (adjusted for loss)3.568.427.20.91003.262.232.91.7100
3. × Dp copy number× 0× 1× 2× 3× 0× 1× 2× 3
4. Dps/100 nuclei (expected)068.454.42.7125.5062.265.85.1133.1
5. % nuclei (observed)23.258.017.51.3(69)61.031.47.60(105)
6. × Dp copy number× 0× 1× 2× 3× 0× 1× 2× 3
7. Dps/100 nuclei (observed)058.035.04.097031.415.2046.7
8. Univalents/100 nuclei(Line 7 × 1/DE)125.5(Line 7 × 1/DE)60.4
9. Trivalents/100 nuclei00000034.016.30.5751.0
10. Quadrivalents/100 nuclei00000009.81.010.8
  • All values are per 100 nuclei (or per 100 males—line 1). Line 1: Census of Dp1187 and Dp62 stocks; percentages of y+ males carrying 0, 1, 2, or 3Dps (see materials and methods). Total males tested are in parentheses. Line 2: Expected frequencies (ef) of 0, 1, 2, and 3Dp spermatocytes obtained from the corresponding frequencies of males (f) in line 1 by adjusting for a premeiotic loss frequency of 5% for each Dp using the binomial formula. Thus ef(3Dp spermatocytes) = f(3Dp males) × (0.95)3, and ef(2Dp spermatoctes) = (f(2Dp males × (0.95)2) + (f(3Dp males) × 3 × (0.95)2 × 0.05)), etc. Line 3: Number of Dp copies in each genotype. Line 4: Expected numbers of Dps in spermatocytes of each class, obtained by multiplying expected spermatocyte frequencies in line 2 by Dp copy number (line 3). Line 5: Observed distribution of nuclei with 0, 1, 2, or 3 univalents. Numbers in parentheses are total numbers of nuclei scored. Line 6: Number of Dp copies in each genotype. Line 7: Observed numbers of univalents in spermatocytes of each class, obtained by multiplying univalent frequencies in line 5 by Dp copy number (line 6). Line 8: Corrected total univalents obtained by multiplying observed total univalents (line 7) by 1/DE. DE (detection efficiency) = observed/expected univalents per nucleus (Dp1187) = 97/125.5 = 0.773. Lines 9 and 10: Expected distributions of trivalents and quadrivalents based on expected Dp62 frequency distribution (line 2) and Dp62 association frequency (AF). AF = 1 − (observed/expected univalents per 100 spermatocytes) = 1 − 60.4/133.3 = 0.547. Thus trivalents/1Dp spermatocytes = 62.2 × 0.547 = 34.0; trivalents/2 Dp spermatocytes = 32.9 × 0.547 × 0.453 × 2 = 16.3; etc. In line 10, expected numbers of quadrivalents and quintivalents are combined.