TABLE 1

Fold difference for multiple D. melanogaster and D. virilis strains

PIDAPI
Strain no.2C ± SE16C/2C ± SE2C ± SE (A:T corrected)16C/2C ± SE
D. melanogaster
Bl 20571.01 ± 0.096.15 ± 0.291.67 ± 0.01 (1.25)6.06 ± 0.04
Bl 14951.00 ± 06.30 ± 0.031.00 ± 0.07 (0.99)9.31 ± 0.13
Bl 44550.99 ± 0.016.09 ± 0.011.19 ± 0.05 (1.06)8.83 ± 0.10
Bl 65991.32 ± 0.035.49 ± 0.171.29 ± 0.05 (1.10)9.17 ± 0.17
Bl 17850.97 ± 0.01 (0.98)10.65 ± 0.07
Bl 5761.13 ± 0.08 (1.04)9.06 ± 0.08
Bl 16331.08 ± 0.06 (1.02)8.85 ± 0.03
H2AvD-GFP1.11 ± 0.02 (1.03)9.09 ± 0.10
Bl 42691.12 ± 0.04 (1.03)9.01 ± 0.05
Bl 1891.04 ± 0.11 (1.00)9.00 ± 0.14
D. virilis
15010-1051.001.97 ± 0.034.44 ± 0.072.71 ± 0.01 (1.64)6.12 ± 0.03
15010-1051.452.09 ± 0.044.73 ± 0.042.54 ± 0.03 (1.58)6.08 ± 0.02
15010-1051.462.25 ± 0.08 (1.47)5.87 ± 0.01
15010-1051.871.78 ± 0.064.57 ± 0.022.38 ± 0.15 (1.52)5.86 ± 0.03
24651.70 ± 0.305.40 ± 1.162.34 ± 0.11 (1.50)5.21 ± 0.02
  • 2C and 16C/2C values were obtained for multiple strains of D. melanogaster and D. virilis using either PI or DAPI dyes in flow cytometric measures of the genome size of ovarian follicle cell nuclei. All values represent averages of three biological replicates, except for D. melanogaster Bl 1495 and Bl 2057, which were measured in four and six biological replicates, respectively. Standard error (±SE) is shown for each value. DAPI values corrected for A:T bias fluorescence as described in Figure 2A and in the materials and methods are shown in parentheses (A:T corrected). Note that, before bias correction, the DAPI values for D. virilis are much higher than the PI 2C values whereas this dye effect is minimal in D. melanogaster 2C values. This reflects a greater total A:T content in D. virilis.