TABLE 2

Classification and validation of differentially expressed genes

GroupExpression in zmet2-m1 vs. wild typeNo. of genesValidated genesaGenes tested in W22bW22 similar to B73cW22 similar to Mo17dW22 similar to bothe
1upUpregulated52 of 22002
1downDownregulated72 of 22002
2upUpregulated234 of 4826NA
2downDownregulated249 of 10413NA
3upUpregulated157 of 8101NA
3downDownregulated62 of 2615NA
4upUpregulated121 of 1110NA
4downDownregulated161 of 1110NA
5upUpregulated60 of 1220NA
5downDownregulated122 of 2000NA
  • a Microarray expression patterns were validated by semiquantitative RT–PCR for wild-type and zmet2-m1 mutant plants in both B73 and Mo17 genetic backgrounds.

  • b Expression was tested in W22 and W22 zmet2-m1 isolines.

  • c Expression differences in W22 relative to W22 zmet2-m1 were similar to the expression pattern observed in B73 and B73 zmet2-m1.

  • d Expression differences in W22 relative to W22 zmet2-m1 were similar to the expression pattern observed in Mo17 and Mo17 zmet2-m1.

  • e Gene expression response in W22 zmet2-m1 plants was similar to the response observed in both B73 and Mo17 genetic backgrounds.