TABLE 3

Two-hybrid interactions between Shs1 and other septins

DBD fusion
AD fusionCdc3Cdc10Cdc11Cdc12Shs1Control
Shs11.150.521.9662.070.030.07
Control0.030.050.070.030.030.06
  • AD-Shs1 means pM-25. DBD fusion plasmids are LexA DBD-Cdc3 (pM-15), LexA DBD-Cdc10 (pM-16), LexA DBD-Cdc11 (pM-17), LexA DBD-Cdc12 (pM-19), and LexA DBD-Shs1 (pM-20). Each pair of plasmids were introduced into J21, and β-galactosidase activity (units) was determined as described in materials and methods.