TABLE 2

Vectors and plasmids used in this study

PlasmidCharacterVectorReference
pGFP316CEN URA3 GFPOur stock
pYO326URA3Ohya et al. (1991)
pJJ215HIS3Jones and Prakash (1990)
pJJ242URA3Jones and Prakash (1990)
pJJ250LEU2Jones and Prakash (1990)
pJJ280TRP1Jones and Prakash (1990)
pYM116ATRP1 lexA-DBDOur stock
pYM116BTRP1 lexA-DBDOur stock
pACTLEU2 ADThis study
pGAD-C1LEU2 ADJames et al. (1996)
pTS910CUCEN URA3 GFPOur stock
pTS910ITTRP1 GFPOur stock
pTS911CTCEN TRP1 PGAL1-5xHAOur stock
pGAL-GFPPGAL1-GFPOur stock
pBluescript II KS+TOYOBO
pM-1CEN URA3 CDC3-GFP (−1000 to +1560)pGFP316Iwase and Toh-e (2001)
pM-2CEN URA3 CDC10-GFP (−1000 to +968)pGFP316Iwase and Toh-e (2001)
pM-3CEN URA3 CDC11-GFP (−1000 to +1244)pGFP316Iwase and Toh-e (2004)
pM-4CEN URA3 CDC12-GFP (−1000 to +1223)pGFP316Iwase and Toh-e (2001)
pM-7URA3 SHS1-GFP (+601 to +1655)pJJ242Iwase et al. (2004)
pM-9URA3 CDC3 (−1000 to +1560)pYO326This study
pM-10URA3 CDC10 (−1000 to +968)pYO326This study
pM-11URA3 CDC11 (−1000 to +1244)pYO326This study
pM-12URA3 CDC12 (−1000 to +1223)pYO326Iwase and Toh-e (2001)
pM-13URA3 SHS1 (−1600 to +1653)pYO326This study
pM-15TRP1 lexA-DBD-CDC3 (+1 to +1560)pMY116BIwase and Toh-e (2001)
pM-16TRP1 lexA-DBD-CDC10 (+3 to +968)pMY116BIwase and Toh-e (2001)
pM-17TRP1 lexA-DBD-CDC11 (+1 to +1244)pMY116AIwase and Toh-e (2001)
pM-19TRP1 lexA-DBD-CDC12 (+1 to +1223)pMY116BIwase and Toh-e (2001)
pM-20TRP1 lexA-DBD-SHS1 (+1 to +1655)pMY116AIwase and Toh-e (2001)
pM-25LEU2 AD-SHS1 (+1 to +1655)pACTThis study
pM-26LEU2 AD-SHS1 (+1 to +1655)pGAD-C1This study
pM-30CDC10 (−1000 to +968)pBluescript II KS+This study
pM-31cdc10Δ∷URA3 (−1000 to −141, +679 to +968)pM-30This study
pM-32cdc10Δ∷LEU2 (−1000 to −141, +679 to +968)pM-30This study
pM-33CDC11 (−1000 to +1244)pBluescript II KS+This study
pM-34cdc11Δ∷TRP1 (−1000 to −25, +571 to +1244)pM-33This study
pM-37SHS1 (−460 to +1655)pBluescript II KS+This study
pM-38shs1Δ∷HIS3 (−460 to −38, +1300 to +1655)pM-37This study
pM-91URA3 PGAL1 GFP-SHS1(+1 to +1655)pGAL-GFPThis study
pM-92TRP1 PGAL1-CDC10-5xHA (+1 to +966)pTS911CTThis study
pM-93LEU2 AD SHS1 (+1 to +271)pGAD-C1This study
pM-94LEU2 AD-SHS1 (+271 to +955)pGAD-C1This study
pM-95LEU2 AD-SHS1 (+1 to +760)pGAD-C1This study
pM-96LEU2 AD-SHS1 (+140 to +1300)pGAD-C1This study
pM-98LEU2 AD-SHS1 (+1 to +1557)pGAD-C1This study
pM-99HIS3 SHS1 (+756 to +1557)pJJ215This study
pM-101CEN URA3 SHS1-GFP (−1600 to +1653)pTS910CUThis study
pM-102CEN URA3 SHS1-GFP (−1600 to +1557)pTS910CUThis study
pM-108TRP1 MYO1-GFP (+4000 to +5784)pTS910ITThis study
pM-109LEU2 AD-SHS1 (+1 to +1557)pACTThis study
pM-2001LEU2 AD-SHS1 (+1 to +1062)pACTThis study
r26-5LEU2 AD-SHS1 (+932 to +1656)pACTThis study
IQG1pUG35CEN URA3 MET25p-IQG1-yEGFP3pUG35Wagner et al. (2002)