TABLE 3

Mutant SME-1 genes selected in the presence or absence of ampicillin

5′UTRSignal sequenceMature sequence
Spontaneous
Cp.1aF19b
Cp.2 (2)cIS1d
Cp.3IS1
Cp.4IS1
CpAp.1e (4)N20Y
CpAp.2 (2)IS5
CpAp.3IS10
Library
Cp.5S2PK273E
Cp.6L14b
CpAp.4Δa73S2AQ128R
CpAp.5 (3)N20S
CpAp.6K4RF19LS62P
CpAp.7F19L
CpAp.8 (2)t121ca124gF19L
  • a Cp clones isolated under chloramphenicol selection.

  • b STOP codon.

  • c Clones isolated multiple times are indicated by number in parenthesis.

  • d Occurance of insertion sequence as described in text.

  • e CpAp clones isolated under chloramphenicol plus 100 μg/ml ampicillin selection.