TABLE 6

alx-1(0) suppresses the enhancement of glp-1(ts) by ego-2 in the germline

GenotypeBrood size (N)% Glp-1 sterile (n)
ego-2(RNAi)192 ± 6 (3)0 (575)
alx-1(0)234 ± 6 (19)0.1 (4453)
unc-36 glp-1(bn18)171 ± 9 (11)1.0 (1877)
unc-36 glp-1(bn18) alx-1(0)178 ± 7 (10)0.1 (1783)
ego-2(RNAi); alx-1(0)186 ± 3 (6)0 (1114)
ego-2(RNAi); unc-36 glp-1(bn18)a168 ± 7 (11)27 (1856)
ego-2(RNAi); unc-36 glp-1(bn18) alx-1(0)a177 ± 8 (8)11 (1413)
  • All tests were done at 20°. “Glp-1 sterile” is as described in the Table 1 legend. N, number of broods scored; n, number of animals scored.

  • a Two independent RNAi experiments were done. We consistently observed reduced enhancement of glp-1 in alx-1(0) vs. alx-1(+) animals.