TABLE 1

The loss of ego-2 function enhances glp-1(bn18ts) in the germline

GenotypeAdults/brood (N)% Glp-1 sterile (n)
glp-1(bn18ts)191 ± 11 (5)1.4 (957)
unc-75190 ± 10 (6)0 (1138)
ego-2(om33) unc-75192 ± 11 (6)0 (1149)
unc-75; glp-1(bn18)135 ± 3 (12)0.1 (1620)
ego-2(om33) unc75; glp-1(bn18)90 ± 12 (10)8.5 (896)
ego-2(om33/tm2272)28 ± 3 (14)0 (398)
ego-2(om33/tm2272); glp-1(bn18)aND88 (175)
ego-2(RNAi)b190 ± 8 (12)0 (2277)
ego-2(RNAi);unc-32 glp-1(bn18ts)b,c134 ± 7 (31)45 (4160)
  • Experiments were done at 20°. N, number of broods scored; n, number of animals scored; ND, not determined. “Glp-1 sterile” is the percentage of germlines in which all germ cells had prematurely exited mitosis, entered meiosis, and differentiated. Number following the “±” is the standard error of the mean.

  • a Animals were obtained as progeny of ego-2(om33) unc-75/ego-2(tm2272); glp-1(bn18/+) mothers (whose average brood size was 36 ± 4.5). A total of 321 progeny (nine broods) were scored; 47/214 ego-2(om33) unc-75/ego-2(tm2272) animals were Glp-1 sterile, which is 88% (47/53) of the expected number of glp-1(bn18/bn18) animals. In addition, 8/107 ego-2(om33) unc-75 animals were Glp-1 sterile, which is 30% (8/27) of the expected number of glp-1(bn18/bn18) animals.

  • b Data are the average of four independent feeding experiments.

  • c A similar degree of enhancement was observed in the absence of the unc-32 marker (data not shown).