TABLE 7

Effects of grandpaternally inherited aubergine mutations on cytotype-mediated repression of P excisions from snw in the male germline

Non-Curly sons testedCurly sons tested
Mother's genotypeaNo. of vialsNo. of fliesExcision rate ± SEbNo.of vialsNo. of fliesExcision rate ± SEb
w snw/+; piwi1/Cy Roi257470.518 ± 0.020298370.583 ± 0.021
w snw/+; aubΔP-3a/Cy Roi204450.500 ± 0.031214890.587 ± 0.031
w snw/+; aubQC42/Cy Roi237220.495 ± 0.026257510.571 ± 0.025
TP5 w snw/+; piwi1/Cy Roi293930.216 ± 0.031303430.287 ± 0.037
TP5 w snw/+; aubΔP-3a/Cy Roi287320.608 ± 0.032273980.512 ± 0.040
TP5 w snw/+; aubQC42/Cy Roi256140.461 ± 0.030297820.406 ± 0.033
  • a These flies were created by crossing w snw; piwi1/Cy Roi or TP5 w snw; piwi1/Cy Roi females to +; mutation/CyO males, where the mutation was piwi1, aubΔP-3a, or aubQC42. They were crossed to males homozygous for the H(hsp/CP)2 transgene, and their non-Curly and Curly sons that had orange (rather than red) eyes and weak singed (rather than wild-type) bristles—that is, that carried the w and snw alleles on the X chromosome and the H(hsp/CP)2 transgene on chromosome 2—were tested for P excisions. Because the w mutation is tightly linked to the left X telomere, it could be used as a marker for the presence of TP5. The non-Curly sons were (TP5) w snw; mutation/H(hsp/CP)2; that is, they carried the aub or piwi mutation, whereas the Curly sons, which were (TP5) w snw; Cy Roi/H(hsp/CP)2, did not.

  • b Average unweighted excision rate [(sn+ + sne)/(snw + sn+ + sne)] ± standard error.