TABLE 3

Overview of the phenotype of the isolated class II mutant lines

Clones at the distal tipClones at the proximal regionAnalyzed clones% at the distal tipLethal line
2L 31940770.0Yes
2L 17480330.0Yes
2L 16820550.0Yes
2L 29830110.0Yes
2L 28340330.0Yes
2L 27980770.0Yes
2L 26863232611.5Yes
2L 33283212412.5Yes
2L 17384151921.1No
  • Class II mutant lines displayed migration phenotypes meeting our screening criteria (<40% of MARCM clones reaching the air sac primordium distal tip). However, class II mutant lines also displayed MARCM clones of reduced size (see also Figure 3, E and F). We recovered nine strains meeting this criterion (see also Table 1). For each line, numbers refer to the amount of MARCM clones observed at the distal tip of the air sac primordium (column 2) and in the proximal region (column 3), to the total number of observed clones (column 4), and to the percentage of MARCM clones localized at the distal tip of the air sac primordium (column 5; Figure 3, E and F). We recovered eight homozygous lethal lines and one homozygous viable line.