TABLE 1

Bacterial strains and plasmids

Strain or plasmidGenotypes and relevant characteristicsSource or reference
P. fluorescensa
    SBW25Wild-type strain isolated from sugar beetThompson et al. (1995)
    SBW25-lacZSBW25 carrying ‘lacZ marker in a phage locusX.-X. Zhang and P. B. Rainey
    PBR800hutF∷Ω or pflu0358∷Ω, insertion at nucleotide 423, SpRThis study
    PBR801ΔhutH1/H2This study
    PBR802ΔhutH1This study
    PBR803ΔhutH2This study
    PBR804hutC∷Ω, insertion at nucleotide 266, SpRThis study
    PBR805ΔhutCThis study
    PBR806ΔhutDThis study
    PBR807ΔhutCDThis study
    PBR808ΔrpoNJ. Jones and G. Preston
    PBR809ΔcbrAThis study
    PBR810ΔcbrBThis study
    PBR811DUP(hutC-hutF)∷pUIC3, the hutF-‘lacZ fusion strain of wild-type SBW25, TcRThis study
    PBR812DUP(hutF-hutC)∷pUIC3, the hutC-‘lacZ fusion strain of wild-type SBW25, TcRThis study
    PBR813DUP(hutD-hutU)∷pUIC3, the hutU-‘lacZ fusion strain of wild-type SBW25, TcRThis study
    PBR814DUP(hutI-hutG)∷pUIC3, the hutG-‘lacZ fusion strain of wild-type SBW25, TcRThis study
    PBR815hutU∷Ω, insertion at nucleotide 633, SpRThis study
    PBR816hutC∷Ω DUP(hutF-hutC)∷pUIC3, hutC-‘lacZ, SpR, TcRThis study
    PBR817hutC∷Ω DUP(hutD-hutU)∷pUIC3, hutU-‘lacZ, SpR, TcRThis study
    PBR818hutC∷Ω DUP(hutI-hutG)∷pUIC3, hutG-‘lacZ, SpR, TcRThis study
    PBR819hutU∷Ω DUP(hutI-hutG)∷pUIC3, hutG-‘lacZ, SpR, TcRThis study
    PBR820ΔhutH1/H2 DUP(hutC-hutF)∷pUIC3, hutF-‘lacZ, TcRThis study
    PBR821ΔhutH1/H2 DUP(hutF-hutC)∷pUIC3, hutC-‘lacZ, TcRThis study
    PBR822ΔhutH1/H2 DUP(hutD-hutU)∷pUIC3, hutU-‘lacZ, TcRThis study
    PBR823ΔhutC DUP(hutD-hutU)∷pUIC3, hutU-‘lacZ, TcRThis study
    PBR824ΔhutD DUP(hutD-hutU)∷pUIC3, hutU-‘lacZ, TcRThis study
    PBR825ΔhutCD DUP(hutD-hutU)∷pUIC3, hutU-‘lacZ, TcRThis study
    PBR832hutU∷Ω DUP(hutD-hutU)∷pUIC3, hutU-‘lacZ, TcRThis study
Plasmid
    pRK2013Helper plasmid, Tra+, KmRDitta et al. (1980)
    pCR2.1Cloning vector, ApR, KmRInvitrogen
    pCR8/GW/TOPOCloning vector, SpRInvitrogen
    pHP45Ω-SpSource of the Ω-Sp cassette, SpRFellay et al. (1987)
    pME6010Broad-host-range cloning vector, TcRHeeb et al. (2000)
    pME6010-hutH1hutH1 construct for complementation, TcRThis study
    pME6010-hutH2hutH2 construct for complementation, TcRThis study
    pUIC3Integration vector with promoterless ‘lacZ, Mob+, TcRRainey (1999)
    pUIC3-2pUIC3∷(hutD-hutF), the hutF-‘lacZ fusion plasmid, TcRThis study
    pUIC3-3pUIC3∷(hutF-hutD), the hutC-‘lacZ fusion plasmid, TcRThis study
    pUIC3-8pUIC3∷(hutD-hutU), the hutU-‘lacZ fusion plasmid, TcRThis study
    pUIC3-12pUIC3∷(hutI-hutG), the hutG-‘lacZ fusion plasmid, TcRThis study
  • a The hut-‘lacZ fusion strain was constructed by cloning the DNA fragment into the delivery vector pUIC3 followed by integration into the hut locus by insertion–duplication. Thus the hut gene function was not affected.