TABLE 1

Marker segregation in F2 lines

F2 linesc
MarkeraPrimersbFragment size (bp)% positiveP (1:1)P (3:1)
H1See text.35955.50.2726.65E-06
H2See text.25048.60.7821.11E-09
AF1aE+AT/M+GA25849.20.8672.42E-09
AF1bE+AT/M+GA24350.80.8672.41E-08
AF2E+AT/M+GG23142.50.1366.58E-14
AF3aE+TA/M+GG23354.20.4021.54E-06
AF3bE+TA/M+GG22745.80.4021.57E-11
AF4E+TA/M+GG25650.80.8672.41E-08
AF5aE+CG/M+AG25740.00.0466.32E-16
AF5bE+CG/M+AG18460.00.0465.32E-04
AF6E+CG/M+AG21857.70.1246.41E-05
AF7E+AT/M+GA19053.00.5483.79E-07
AF8E+AT/M+GG10048.60.7821.11E-09
AF9E+AT/M+GG18043.10.1671.73E-13
AF10E+CG/M+AG9047.30.5831.47E-10
AF11E+ACC/M+G28047.20.5791.41E-10
AF12E+ACC/M+G23049.40.9123.59E-09
AF22aE+CAA/M+TA20547.20.5791.41E-10
AF22bE+CAA/M+TA22052.80.5792.87E-07
AF23aE+ACG/M+CTT9347.80.6573.24E-10
AF23bE+ACG/M+CTT7952.20.6571.44E-07
AF24aE+CG/M+CTT9350.30.9551.14E-08
AF24bE+CG/M+CTT7949.70.9555.28E-09
AF25aE+CA/M+GA15447.00.5439.47E-11
AF25bE+CA/M+GA15553.00.5433.94E-07
  • a Markers labeled “a” and “b” after the same numerical designation are allelic.

  • b E and M stand for universal AFLP primers (E: GACTGCGTACCAATTC; M: GATGAGTCCTGAGTAA). Sequences of H1 and H2 primers are listed in materials and methods.

  • c P represents the P-value of the chi-squared goodness-of-fit test for 1:1 or 3:1 segregation.