TABLE 2

Preferential elongation of the budded part of the cell cycle in SIC1-0P cells

MeasureSIC1 genotypeMean ± SD (n)Wild type vs. SIC1-0P (t-test)
Unbudded period, daughtersWild type49 ± 27 (13)P < 0.001
SIC1-0P16 ± 6 (29)
Budded period, daughtersWild type71 ± 17 (10)P < 0.001
SIC1-0P119 ± 40 (14)
Cell cycle, daughtersWild type119 ± 28 (10)P < 0.25
SIC1-0P134 ± 41 (14)
Unbudded period, mothersWild type25 ± 8 (15)P < 0.001
SIC1-0P14 ± 9 (27)
Budded period, mothersWild type65 ± 17 (10)P < 0.001
SIC1-0P103 ± 19 (14)
Cell cycle, mothersWild type90 ± 16 (10)P < 0.005
SIC1-0P116 ± 18 (10)
  • MYO1-GFP cells, either SIC1-wt or SIC1-0P, were analyzed by time-lapse microscopy, and the times of budding and Myo1 ring formation and cytokinesis, as marked by Myo1 ring closure and disappearance (Bi et al. 1998), were scored manually. The unbudded period was defined as the time from cytokinesis to budding, and the budded period as the time from budding to cytokinesis. The total cell cycle was the time from one cytokinesis to the next. Data for mothers and daughters were analyzed separately.