TABLE 1

CLN2pr-GFP expression and other statistics from quantitative time-lapse fluorescence microscopy

MeasureSIC1 genotypeMean ± SD (n)Wild type vs. SIC1-0P (t-test)
CLN2pr-GFP peak amplitude (AU)Wild type1.04 ± 0.23 (57)P < 0.001
SIC1-0P1.46 ± 0.40 (65)
CLN2pr-GFP peak width (in minutes)Wild type47 ± 5.6 (57)P < 0.001
SIC1-0P54 ± 5.6 (70)
Budding to CLN2pr-GFP peak (in minutes)Wild type21 ± 8 (88)P < 0.001
SIC1-0P27 ± 6 (89)
Cell size at budding (pixels)Wild type1983 ± 311 (104)P < 0.001
SIC1-0P2914 ± 587 (127)
Mother-cell cycle time (in minutes)Wild type95 ± 19 (59)P < 0.001
SIC1-0P121 ± 25 (85)
Bud long axis/short axis length ratio (60 min after budding)Wild type1.1 ± 0.06 (50)P < 0.001
SIC1-0P1.7 ± 0.389 (81)
  • Data were obtained as described in Bean et al. (2006). Nine-hour recordings (images every 3 min), starting with seven mutant and six wild-type founder cells containing CLN2pr-GFP were made, images were automatically segmented and assigned background-subtracted GFP signal values for each cell body, and bud emergence timing and mother–daughter relationships were assigned using the graphical user interface (Bean et al. 2006). Computations are described in materials and methods. Bud dimensions were determined using ImageProPlus on randomly selected cells 60 min after bud emergence. AU, arbitrary units.