TABLE 5

Regulation of YLL055w by different sulphur regulatory trans-factors

Strain background− Methionine+ Methionine
Wild type (ABC 734)8.4 ± 0.43.1 ± 0.4
Wild type (ABC 1381)17.8 ± 1.15.6 ± 1.6
met4Δ (ABC1081)3.7 ± 1.15.6 ± 1.5
met28Δ (ABC 1080)212.5 ± 7.56.2 ± .08
cbf1Δ (ABC 1082)15.0 ± 0.64.6 ± 2.6
met31Δ (ABC 1031)15.0 ± 0.74.3 ± 0.25
met32Δ (ABC 1032)2.9 ± 0.21.6 ± 0.2
  • The pYLL055w-600 multicopy plasmid bearing a 600-bp upstream region of YLL055w fused upstream of the β-galactosidase gene was transformed into strains deleted for the different sulphur regulatory trans-factors and their respective wild-type strains. The transformants were grown in the presence and absence of methionine and the β-galactosidase enzyme activity was measured. Data are shown as mean ± SD (n = 3).