TABLE 1

DNA repair genes included in this study

S. pombe geneS. cerevisiae closest homologFunction in S. pombe
lig4+DNL4ATP-dependent DNA ligase involved in NHEJ (Baumann and Cech 2000)
pku70+YKU70DNA-binding protein involved in DNA repair and telomere maintenance (Baumann and Cech 2000)
exo1+EXO15′ → 3′ exonuclease (XP-G family) involved in several DNA repair pathways such as MMR (Rudolph et al. 1998) and possibly HR (Tomita et al. 2003)
rad2+RAD27ssDNA endonuclease (XP-G family) homologous to FEN-1; involved in mismatched DNA repair (Kunz and Fleck 2001) and telomere maintenance (Dahlen et al. 2003)
rad13+RAD2ssDNA endonuclease (XP-G family) involved in NER (Kunz and Fleck 2001)
spac12g12.16c+RAD27Member of the XP-G family of nucleases with unknown function
rad22+RAD52Annealing of complementary single-stranded DNA; required for HR (Hegde et al. 1996; van den Bosch et al. 2001, 2002)
rhp51+RAD51AAA ATPase with strand displacement activity involved in HR and telomere maintenance (Kibe et al. 2003)
rqh1+SGS1DEAD/DEAH box DNA helicase (RecQ family) involved in HR-dependent DSB repair (Hegde et al. 1996)
msh2+MSH2DNA mismatch repair protein (MutS homolog) required for MMR (Rudolph et al. 1998)
pms1+PMS1DNA mismatch repair protein (MutL homolog) required for MMR (Rudolph et al. 1998)
swi10+RAD10ssDNA repair endonuclease (ERCC1 family); forms a complex with Rad16 and is involved in NER (Carr et al. 1994; Rodel et al. 1997)
rad16+RAD1ssDNA repair endonuclease (XP-F family); forms a complex with Swi10 and is involved in NER (Carr et al. 1994; Rodel et al. 1997)
pol4+POL4PolX DNA polymerase with gap-filling activity; combines properties of mammalian DNA polymerase β, μ, and λ; possibly involved in BER and NHEJ (Gonzalez-Barrera et al. 2005)
rad50+RAD50AAA ATPase involved in sister-chromatid recombination (Hartsuiker et al. 2001) and intermolecular NHEJ (Decottignies 2005)