TABLE 3

Percentage of donor mtDNA persisting in intergeneric NT embryos

Donor cell1 cell2 cell3 cell4 cell8 cell12 cell16 cell20 cell
mtDNA+0.00 (n = 4)0.00 (n = 1)0.00 (n = 1)0.00 (n = 3)0.00 (n = 1)0.00 (n = 3)0.00 (n = 1)ND
0.03 (n = 1)19.99 (n = 1)
mtDNAR0.00 (n = 9)0.00 (n = 3)ND0.00 (n = 3)ND0.00 (n = 3)ND0.00 (n = 2)
0.06 (n = 1)3.42 (n = 1)
  • The percentage of donor mtDNA present in individual embryos was determined by AS real-time PCR. Each reaction was run in triplicate and performed at least twice with a minimum sensitivity of 0.01% for the donor allele (see Figure 7). Values of 0.00 are equivalent to homoplasmy; other values represent heteroplasmy. The number of embryos for each stage is given in parentheses (n). ND, not determined/no data.