TABLE 2

Fatty acid composition of lipid fractions from wild-type and fat-6;fat-7 double mutants

Fatty acidWild-type TAGfat-6;fat-7 TAGWild type PLfat-6;fat-7 PL
14:02.3 ± 0.32.4 ± 0.60.7 ± 0.10.5 ± 0.1
16:04.0 ± 2.52.2 ± 1.94.1 ± 0.51.8 ± 1.3*
18:03.6 ± 0.120.2 ± 3.1**9.3 ± 1.823.5 ± 4.0*
Total saturated9.924.814.125.8
16:12.3 ± 0.23.20.9 ± 0.13.0 ± 0.1***
18:1Δ96.0 ± 0.1—***2.2 ± 0.2—***
18:1Δ1114.6 ± 2.616.8 ± 0.915.0 ± 1.726.0 ± 3.9*
Total MUFA21.820.016.829.0
18:23.0 ± 0.6—*4.6 ± 0.5—***
20:32.5 ± 0.3—**6.8 ± 0.5—***
20:4n-60.8 ± 0.42.5 ± 0.4—*
20:4n-31.5 ± 0.3—*9.4 ± 0.1—***
20:53.1 ± 0.5—**22.0 ± 1.1—***
Total PUFA11.546.1
18:1 Δ132.2 ± 0.2**1.7 ± 0.1***
18:3 Δ8,11,140.8 ± 0.78.3 ± 0.9***
18:4 Δ5,8,11,140.5 ± 0.55.6 ± 0.8**
18:4 Δ8,11,14,170.5 ± 0.56.3 ± 1.3***
Total unusual4.021.9
C15iso5.7 ± 0.22.0 ± 0.5**1.8 ± 0.1—***
C17iso3.2 ± 0.22.1 ± 0.4*2.6 ± 0.10.9 ± 0.3**
Total branched8.94.14.50.9
17Δ28.8 ± 0.631.1 ± 2.46.7 ± 0.710.6 ± 1.7*
19Δ16.8 ± 1.212.2 ± 1.5*6.8 ± 0.57.7 ± 2.9
Total cyclopropane45.643.313.518.3
  • Data are weight percentages of total fatty acids (mean ± SD) of lipid extractions from three independently grown nematode populations measured by gas chromatography. 17Δ, 9,19-methylenehexadecanoic acid; 19Δ, 11,12-methyleneoctadecanoic acid; TAG, triacylglyceride fraction; PL, phospholipid fraction. Dashes indicate fatty acids <0.5%. Values determined to be significantly different from wild-type lipid fractions using an unpaired t-test are *P < 0.05, **P < 0.001, and ***P < 0.0001.