TABLE 1

Fatty acid composition of wild-type and Δ9 desaturase double mutants

Fatty acidWild typefat-5;fat-7fat-5;fat-6fat-6;fat-7
14:01.6 ± 0.12.3 ± 0.1***1.9 ± 0.31.4 ± 0.3
16:05.5 ± 0.57.1 ± 0.6***6.4 ± 0.6*1.6 ± 0.3***
18:08.5 ± 1.09.9 ± 1.2*9.4 ± 0.522.2 ± 1.3***
Total saturated15.619.317.725.2
16:11.4 ± 0.11.2 ± 0.11.1 ± 0.1**3.4 ± 0.8***
18:1Δ93.2 ± 0.24.2 ± 0.4**3.2 ± 0.2—***
18:1Δ1114.3 ± 1.313.5 ± 0.612.6 ± 1.3*24.0 ± 1.6***
Total MUFA18.918.916.927.4
18:23.1 ± 0.13.5 ± 0.3*3.0 ± 0.3—***
20:34.4 ± 0.44.1 ± 0.33.9 ± 0.5—***
20:4n-62.0 ± 0.41.7 ± 0.11.5 ± 0.2—***
20:4n-35.0 ± 0.64.5 ± 0.64.4 ± 0.6—***
20:511.7 ± 1.711.0 ± 1.410.2 ± 1.2—***
Total PUFA26.224.823.0
18:1 Δ132.0 ± 0.3***
18:3 Δ8,11,148.0 ± 2.0***
18:4 Δ5,8,11,144.1 ± 0.3***
18:4 Δ8,11,14,173.7 ± 0.3***
Total unusual17.8
C15iso3.9 ± 0.33.6 ± 0.24.3 ± 0.40.7 ± 0.3***
C17iso3.1 ± 0.32.4 ± 0.2*3.5 ± 0.2*1.5 ± 0.6***
Total branched7.06.07.42.2
17Δ19.9 ± 0.919.1 ± 1.020.9 ± 1.818.8 ± 2.1
19Δ13.2 ± 0.911.7 ± 0.7*13.8 ± 0.98.8 ± 1.5*
Total cyclopropane33.130.834.727.6
  • Data are weight percentages (mean ± SD) of four to six independent determinations of total worm fatty acids measured by gas chromatography. 17Δ, 9,19-methylenehexadecanoic acid; 19Δ, 11,12-methyleneoctadecanoic acid. Dash indicates fatty acids <0.5%. Values determined to be significantly different from wild-type worms using an unpaired t-test are *P < 0.05, **P < 0.001, and ***P < 0.0001.