TABLE 1

Summary of genes tested for their regulation of ProX-DHS reporter gene transcription

Chromatin regulators testedDominant effectHomozygous mutants
PcND
PscND
PclND
Su(z)2ND
ScmND
AsxND
HP1ND
Su(var)3-9ND
Pr-set-7ND
PIWIND
GAGA factorND
  • At least two lines of ProX1-DHS or ProX2-DHS were crossed into each genetic background. The transcription level of the GFP reporter gene was measured by real-time RT–PCR and compared with that of a wild-type genetic background. A change less than twofold was scored as negative (−). Transcription was assayed in a heterozygous background for HP1 mutant and PcG mutants and in a homozygous background for all the other mutants. ND, not determined.