TABLE 2

Suppression of meiotic silencing of unpaired loci by Sad-1/Sad-2, Sk-2, and Sk-3

Parent 2
Parent 1a× Wild type× Sad-1Δ or Sad-2RIP× Spore killer-2× Spore killer-3× r(Sk-2)× pr(Sk-2) mod(pr)
act+Silenced, lollipop asci arrested in meiosis INot silenced, normal asciNot silenced, 4:4 asciPartially silenced, 4:4 asciSilenced (no suppression)Silenced (no suppression)
asm-1+Silenced, mostly white ascosporesNot silenced, 8-spored asciPartially silenced, 50% 4:4 asciPartially silenced, 25% 4:4 asciSilencedSilenced
BmlR (mutant β-tubulin)Silenced, all asci blocked in meiosis INot silenced, 8-spored asciNot silenced, 4:4 asciNot silenced, 4:4 asciSilencedSilenced
Bml+-GFPSilenced, asci blocked in meiosis INot silenced, 8-spored asciNot silenced, 4:4 asci for fluorescence and SkNot silenced, 4:4 asci for fluorescence and SkNDND
Dip-1 (Diploid ascospores)Silenced, asci mostly 2-4 sporedNot silenced, 8-spored asciNot silenced, 4:4 asciSilenced, asci mostly 2-4 sporedSilencedSilenced
Dp(VIIR → IL)5936Silenced, barren peritheciaNot silencedNot silenced, 4:4 asciNot silenced, 4:4 asciSilencedSilenced
hH1-GFPSilenced, no fluorescent nuclei in meiosisNot silenced, all nuclei fluoresceNot silenced, nuclei fluoresce, 4:4 asciNot silenced, nuclei fluoresce, 4:4 asciNDND
hH3hH4-1+Silenced, asci showed meiotic defectsNot silencedNot silenced, 4:4 asciNot silenced, 4:4 asciSilencedSilenced
mat A(IL → VR)Silenced, reduced fertilityNot silencedNot silenced, 4:4 asciNot silenced, 4:4 asciSilencedSilenced
mei-3+Silenced, meiotic defects, aborted asciNot silenced, 8-spored asciNot silenced, 4:4 asciPartially silenced, meiotic defectsSilencedSilenced
Round sporeSilenced, round ascosporesNot silenced, normal ascosporesSilenced, round ascospores, 4:4 asciSilenced, round ascospores, 4:4 asciSilencedSilenced
Sk-2 (Spore killer-2)4K:4S ascospores in all asci4K:4S ascospores in all asciNo killing, all 8 ascospores viableAll ascospores killed (mutual killing)Partial killing, mostly 8:0, some 4:4 asciPartial resistance, mostly 4:4 asci
Sk-3 (Spore killer-3)4K:4S ascospores in all asci4K:4S ascospores in all asciAll ascospores killed (mutual killing)No killing, all 8 ascospores viableNDND
  • “Silenced” indicates that the mutation or the inserted gene failed to function during ascus development because of meiotic silencing; “Not silenced” indicates that the mutation or the inserted gene functioned normally because of suppression of meiotic silencing of unpaired loci by Sad-1Δ/Sad-2RIP, Sk-2, or Sk-3; “ND” (no data) indicates that the cross was not examined.

  • a “∷” indicates that an extra copy of a wild-type gene is inserted ectopically at the his-3 locus on LG IR or at the am locus on LG VR.