TABLE 3

Genomic DNA amplification (DNA), DNA methylation (5mC), and RNA levels (RNA) in Sadhu elements in a set of Arabidopsis accessions

Sadhu3-1aSadhu7-2Sadhu6-1
AccessionStock no.DNA5mCRNADNA5mCRNADNA5mCRNA
Br-0CS22628YesYes+NoNTNTYesYes
Bur-0CS22656YesYesNoNTNTYesYes
ColLehleWT-2YesYesYesYesYesYes
CviLehleWT-18YesYes++NoNTNTNoNT
Cvi-0CS22614NoNTNTNoNTNTNoNT
Fei-0CS22645YesYesNoNTNTYesYes
Kn-0CS6762NoNTNTYesYesYesYes
KondaraCS22651YesYesNoNTNTYesNo+++
Kz-1CS22606NoNTNTNoNTNTYesNo+++
N13CS22491YesYesYesYesYesYes
No-0CS6805NoNTNTYesYesNoNT
Po-0CS6839YesYes++NoNTNTYesNo+++
Pro-0CS22649YesYesNoNTNTYesNo+++
Pu2-7CS22592YesYesYesYesYesYes
Ra-0CS22632YesNo+++YesYesYesYes
Tamm-27CS22605NoNTNTNoNTNTYesNo
Ts-1CS22647NoNTNTNoNTNTYesNo
Tsu-1CS22641YesYesYesYesYesYes++
Van-0CS22627YesYesNoNTNTYesYes
Wei-0CS22622YesYesYesYesYesYes
Ws-2CS22659YesYesYesYesYesYes
  • NT, not tested. Genomic DNA amplification uses X1 and Y1 primers (supplemental Table 1 at http://www.genetics.org/supplemental/). DNA methylation is determined by McrBC–PCR using X1 and Y1 primers. RNA levels are detected by RT–PCR: −, none detected; +, low levels; ++, moderate levels; +++, high levels.

  • a Data are from Rangwala et al. (2006).