Completeness of gap repair

TransgeneFly line% offspring with complete gap repair
F (11-kb gap)F1 (X chromosome)100 (12/12)
F1–2 (second chromosome)100 (1/1)
F1–3 (third chromosome)91 (10/11)
F–R (11-kb gap)F–R2 (second chromosome)83 (5/6)
F–R3 (second chromosome)100 (9/9)
F–R4 (second chromosome)100 (1/1)
F–R5 (third chromosome)100 (5/5)
G (28-kb gap)G1 (third chromosome)73 (11/15)
G2 (third chromosome)79 (11/14)
K (48-kb gap)K3.8 (second chromosome)0 (0/6)
J (87-kb gap)J1 (X chromosome)0 (0/11)
  • Numbers represent independent y+ events with a completely copied region in percentage of all y+ events. Completeness of gap repair was analyzed by both genetic and molecular means. F1, F1–2, F1–3, F–R2, F–R3, F–R4, F–R5, G1, G2, K3.8, and J1 are transgenic fly lines for the corresponding constructs F, F–R, G, K, and J. Incomplete in vivo cloning events carry a single large deletion (Figures 2 and 4).