TABLE 2

y+ reconstitution efficiency at different developmental stages

% events/crosses% y+ in total offspring (y+ flies/total no.)
ConstructSexEmbryoFirst instarSecond instarThird instarEmbryoFirst instarSecond instarThird instar
F–R3M62 (31/50)74 (37/50)59 (20/34)23 (22/95)4.6 (201/4390)6.5 (282/4319)4.7 (106/2269)0.6 (40/6870)
F32 (15/47)38 (13/34)40 (14/50)27 (21/79)0.8 (38/4736)1.3 (45/3437)1.5 (40/2687)0.8 (54/6599)
F–R5M10 (5/50)48 (24/50)28 (14/50)11 (9/85)0.8 (35/4421)1.5 (66/4511)0.7 (21/2824)0.16 (10/6218)
F27 (14/51)30 (15/50)45 (20/44)22 (19/86)1.3 (54/4118)1.1 (50/4739)1.4 (47/3320)0.65 (40/6043)
  • DSBs were induced at various developmental stages as indicated (1 hr 38°). F–R3 and F–R5 are transgenic fly lines for the corresponding construct F–R.