TABLE 1

y+ reconstitution efficiency of different constructs and insertions

TransgeneFly lineSex% events/crosses% y+ in total offspring% average efficiency of the constructs (events/crosses)
F1 (11-kb gap)F1 (X chromosome)M100 (19/19)15.7 (147/932)60
F69 (24/35)11.0 (438/3957)
F1–2 (second chromosome)M28 (7/25)0.79 (13/1633)
F25 (1/4)0.37 (1/271)
F1–3 (third chromosome)M52 (26/50)1.95 (59/2958)
F86 (6/7)3.66 (19/500)
F–R (11-kb gap)F–R2 (second chromosome)M12 (4/33)0.45 (9/1980)17
F24 (8/33)0.50 (12/2395)
F–R3 (second chromosome)M23 (22/95)0.58 (40/6870)
F27 (21/79)0.82 (54/6599)
F–R4 (second chromosome)M6 (1/18)0.091 (1/1096)
F13 (2/16)0.50 (6/1173)
F–R5 (third chromosome)M11 (9/85)0.16 (10/6218)
F22 (19/86)0.66 (40/6043)
G (28-kb gap)G1 (third chromosome)M15 (12/80)0.28 (21/7366)13
F13.5 (10/74)0.29 (16/5564)
G2 (third chromosome)M0 (0/38)0 (0/2119)
F23 (9/40)0.29 (9/3018)
K (48-kb gap)K 3.8 (second chromosome)M10 (12/118)0.29 (30/10225)9.5
F9 (9/102)0.32 (28/8518)
J (87-kb gap)J1 (second chromosome)aND2.5 (11/450)0.04 (11/30000)2.5
N (one arm of homology)N2–3ND0.2 (1/500)0.0022 (1/45000)0.2
  • DSBs were generated by the heat-shock-inducible expression (1 hr at 38°) of the I-SceI endonuclease at the early third instar stage. F1, F1–2, F1–3, F–R2, F–R3, F–R4, F–R5, G1, G2, K3.8, J1, and N2–3 are transgenic fly lines for the corresponding constructs F, F–R, G, K, J, and N. M, male; F, female. The average efficiency for each construct was obtained by averaging the efficiency of each insertion of a particular construct. A single tube contains ∼80–120 flies of the relevant genotype. ND, not determined.

  • a For the J and the N construct, three instead of one heat shock (1 hr 38°) was applied on subsequent days. The difference between the sexes was not addressed for the insertions of the J or the N construct.