TABLE 3

Summary of the results obtained from the screen

Times recoveredGenePlasmid nameVectorImproves T(K53R)-YDJ1 growth at 35°?Improves ydj1Δ growth at 30°?
6REC102pCMS125-REC102pRS426
CHS5pSR6pRS316
CHS5pSR23pRS426
MID2-HAp1245YEp352++++++
MID2-GFPp1300pRS314ND+++
5STM1Yep/STM1YEp213a
YLR149cpCMS119-YLR149cpRS426
3RIM1NDNDNDND
SYP1SYP1pRS316pRS316
SYP1SYP1pRS426pRS426++
RPS14aNDNDNDND
2SSA1Yep351-SSA1YEp351 pGPD426 ND++b
SSA1pRS426-GPD-(His)6-SSA1ND+b
EFB1NDNDND
ERP2NDNDND
2SLG1-HAp1657YEp352+++++
2SUS1pSUS1pRS316
CYC8pFW46pRS316+
CYC8pRT81YEp24++++
YBR113wNDNDNDND
1YDR020cNDNDNDND
FAL1NDNDNDND
CIS1pCMS118-CIS1pRS426++
SES1NDNDNDND
1SKN1pSKN1-IVYCp50 pRS416+
THI4pThi4ura3ND
ENP2NDNDND
  • Eight unique plasmids were isolated in the screen that allowed growth of the ydj1Δ yeast-expressing T(K53R)-Ydj1p at 35° upon retransformation, and the number of times each plasmid was identified is indicated. Select individual genes found on each plasmid isolated in the screen were tested for improved growth of T(K53R)-YDJ1 at 35° and ydj1Δ at 30°. Seven genes improved growth at 35° of ydj1Δ yeast-expressing T(K53R)-Ydj1p and four genes improved the slow-growth phenotype of ydj1Δ yeast at 30°. +++, moderate rescue; ++, weak rescue; +, poor rescue. ND, not determined.

  • a Papillae colonies were detected.

  • b ydj1Δ yeast with high-copy SSA1 expression vectors grew more slowly than empty vector controls.