Increased SUC2 expression correlates with increased localization of the gene to the nuclear periphery
| SUC2 expressiona | % cells with peripheral SUC2 ORFb | |||
|---|---|---|---|---|
| Genotype | R (glucosec) | D (no glucosec) | R (glucosec) | D (no glucosec) |
| WT | 2.6 ± 0.2 | 267.2 ± 31.3 | 45 | 74 |
| Δmig1 | 52.8 ± 8.8 | 148.4 ± 1.8 | 60* | 69 |
| Δhxk2 | 65.4 ± 8.2 | 164.1 ± 36.6 | 60** | 72 |
↵a SUC2 expression was measured with invertase assays as described in Neigeborn and Carlson (1984). Error denotes the standard deviation of four determinations.
↵b Percentage of cells where the SUC2 ORF is localized to the outer third of the nucleus. P-values, calculated on the basis of a two-tailed Student's t-test, represent a significant difference in the localization of the ORF in glucose-grown WT cells relative to glucose-grown Δmig1 or Δhxk2 cells. *Significant (P < 0.01); **highly significant (P < 0.0005). At least 115 cells were measured for each condition.
↵c See materials and methods for growth conditions.