Trap type predicted by insertion site position relative to genes
Screen | Independent insertions | % lethal | Protein | Protein?a | Enhancer | Enhancer opposite | No gene |
---|---|---|---|---|---|---|---|
G | 82 | ND | 55 | 0 | 9 | 9 | 9 |
ZCL | 139 | ND | 93 | 2 | 12 | 22 | 10 |
P | 60 | ND | 19 | 1 | 19 | 11 | 10 |
YB | 159 | 33 | 36 | 1 | 54 | 46 | 22 |
YC | 65 | 30 | 43 | 3 | 8 | 7 | 4 |
YD | 314 | 47 | 49 | 10 | 152 | 30 | 73 |
Total | 819 | 295 | 17 | 254 | 125 | 128 | |
Genes | 335 | 115 | 4 | 136 | 82 | NA |
↵a Examination of these insertions showed the potential for splicing the GFP exon to the first exon of a gene and maintaining an open reading frame. RNA analysis on these lines is needed to assign them to protein or enhancer trap designations.