TABLE 1

Phenotypes of HLB368 and IL-7 knockout mice

StrainsHLB368IL-7−/− (von Freeden-Jeffery et al. 1995)IL-7−/− (Rich 1997)
Genomic cloneNA129/SV129/Sv
ES cellsNA129P2/OlaHsd129S6/SvEvTac
StrainB6B6;129129; NIH Black Swiss
Neo cassetteNA++
Thymus and spleenReduced in size and weightReduced in size and weightReduced in size and weight
LNInguinal nodes onlyOneNR
WBCDecreasedDecreasedNR
Cellularity
BMNormalNormalDecreased by 40%
SpleenReduced by 60%Reduced by 85%Reduced by 70%
ThymusReduced by 90%Reduced by 95%Reduced by 95%
B-cell development
EarlyBlocked at Fr. A to B/C transitionBlocked at Fr. C to D or Fr. A to B/C transitionNR
LateDecreased FO subset; B1a and MZ B cells are normal; splenic B cells are CD43 but express activation markers.Decreased FO subset; B1a and MZ B cells are normal; splenic B cells express CD43 and activation markers.NR
T-cell developmentDecreased in numbers; the percentages of CD4 and CD8 cells are normal.Decreased in numbers; the percentages of CD4 and CD8 cells are normal.Decreased in numbers; the percentages of CD4 and CD8 cells are normal.
ReferenceThis studyvon Freeden-Jeffry et al. (1995); Carvalho et al. (2001)Rich (1997)
  • NA, not applicable; NR, not reported.