TABLE 7

Evidence for inversion in crosses heterozygous for easUCLA: suppressed crossing over of IIR markers right of eas

Crossovers
Zygote genotypea and recombination %Singles
ParentalsRegion 1Region 2Doubles
Homozygous normal sequence
 +    ace-1244
un-20   +163
    15
easUCLA heterozygous
 *  +    +262
   un-20   ace-1227
        16
Homozygous normal sequence
 +    trp-3455
ace-1    +375
    11
easUCLA heterozygous
 *  +   trp-3370
   ace-1   +180
      0
Homozygous normal sequence
 +    un-15485
trp-3   +3010
    16
 +    +294
trp-3  un-15404
    10
easUCLA heterozygous
    +  un-15250b
 *  trp-3  +460
    0
 *  +   +370
   trp-3  un-15300
     0
Homozygous normal sequence
 +  +   +241490
pe   fl  un-1518960
   29  19
easUCLA heterozygous
 * +  +   +211600
  pe  fl  un-15221300
   40  0
Homozygous normal sequence
 +   +   +12920
arg-12fl  rip-117950
    33  13
easUCLA heterozygous
 * +   +   +221200
  arg-12fl  rip-123400
     26  0
  • Normal sequence is pe arg-12 un-20 ace-1 eas fl trp-3 un-15 rip-1. Conventions as in Table 2. Distance between markers is approximately as follows in the normal sequence IIR map that uses the point-mutant eas allele JD105: pe (1%, 5%) arg-12 (16%) un-20 (15%) ace-1 (1%) easJD (9%) fl (2%, 6%) trp-3 (10%) un-15 (1%) rip-1.

  • a The eas parent is indicated by an asterisk in crosses heterozygous for easUCLA.

  • b One phenotypically Trp+ Un+ progeny proved to be a pseudowild heterokaryon. This produced trp-3 un-3+ and trp-3+ un-3 progeny when testcrossed.