TABLE 6

Effect of the RIP-defective gene rid on the frequency of stable cya-8 mutations among progeny of crosses involving easUCLA

Progeny
ParentsaStable transparentsbLaggardscTotal
rid homozygous
    rid; easUCLA × rid; easUCLA (2)213300
    rid; easUCLA × rid65212
    rid; easUCLA × rid; sad-212266
Total9 (1)17 (2)778
rid heterozygous
    rid; easUCLA × easUCLA1011154
    rid; easUCLA × WT (2)185143
    rid; easUCLA × sad-215092
Total43 (11)16 (4)389
No ridd
    easUCLA × easUCLA16094
    easUCLA × WT150114
Total31 (15)0208
No easUCLA
    rid × rid (2)16214
    WT × WT01253
Total1 (<1)7 (1)467
  • Numbers in parentheses are percentages.

  • a When the numbers were pooled for progeny from more than one cross, the number of crosses is given in parentheses. WT (wild type): OR23-1VA or ORS-6a. rid (RIP-deficient): suppressor of RIP. sad-2 (suppressor of ascus dominance): suppressor of MSUD.

  • b Stable transparents are expected to result from RIP of duplicated cya-8+.

  • c Laggards are defined as progeny that were classified as transparent 3 days after germination, but that reverted to normal subsequently.

  • d The 19 crosses of easUCLA × easUCLA .or easUCLA × eas+ in Table 1, with 21% transparent progeny, might also be taken as “No rid” controls. The two additional crosses reported here were made because laggards were not recorded as such when the numbers were obtained for Table 1. At that time, most laggards would have been scored as nontransparent, but a few laggards may have been classified as transparent.