Effect of the RIP-defective gene rid on the frequency of stable cya-8 mutations among progeny of crosses involving easUCLA
| Progeny | |||
|---|---|---|---|
| Parentsa | Stable transparentsb | Laggardsc | Total |
| rid homozygous | |||
| rid; easUCLA × rid; easUCLA (2) | 2 | 13 | 300 |
| rid; easUCLA × rid | 6 | 5 | 212 |
| rid; easUCLA × rid; sad-2 | 1 | 2 | 266 |
| Total | 9 (1) | 17 (2) | 778 |
| rid heterozygous | |||
| rid; easUCLA × easUCLA | 10 | 11 | 154 |
| rid; easUCLA × WT (2) | 18 | 5 | 143 |
| rid; easUCLA × sad-2 | 15 | 0 | 92 |
| Total | 43 (11) | 16 (4) | 389 |
| No ridd | |||
| easUCLA × easUCLA | 16 | 0 | 94 |
| easUCLA × WT | 15 | 0 | 114 |
| Total | 31 (15) | 0 | 208 |
| No easUCLA | |||
| rid × rid (2) | 1 | 6 | 214 |
| WT × WT | 0 | 1 | 253 |
| Total | 1 (<1) | 7 (1) | 467 |
Numbers in parentheses are percentages.
↵a When the numbers were pooled for progeny from more than one cross, the number of crosses is given in parentheses. WT (wild type): OR23-1VA or ORS-6a. rid (RIP-deficient): suppressor of RIP. sad-2 (suppressor of ascus dominance): suppressor of MSUD.
↵b Stable transparents are expected to result from RIP of duplicated cya-8+.
↵c Laggards are defined as progeny that were classified as transparent 3 days after germination, but that reverted to normal subsequently.
↵d The 19 crosses of easUCLA × easUCLA .or easUCLA × eas+ in Table 1, with 21% transparent progeny, might also be taken as “No rid” controls. The two additional crosses reported here were made because laggards were not recorded as such when the numbers were obtained for Table 1. At that time, most laggards would have been scored as nontransparent, but a few laggards may have been classified as transparent.