Crossovers | ||||
---|---|---|---|---|

Zygote genotype and recombination % | Singles | |||

Parentals | Region 1 | Region 2 | Doubles | |

+ T(ALS179) | 14 | 0 | ||

cya-8 N | 6 | 1 | ||

5 | ||||

Plus 13 Barren, all cya-8^{+} | ||||

+ adh nic-3 | 287 | 56 | 45 | 2 |

cya-8 + + | — | — | — | — |

15 12 | ||||

Nine crosses pooled | ||||

+ cyt-7 | — | 4 | ||

cya-8 + | — | — | ||

7 | ||||

Plus 109 cya-8, or cyt-7, or cya-8 cyt-7 | ||||

N cyt-7 | 6^{a} | — | ||

T(ALS179) + | — | 0 | ||

0 | ||||

Plus 11 cyt-7 Barren^{a} | ||||

+ adh nic-3 | 19 | 2 | 0 | 0 |

cyt-7 + + | 28 | 2 | 1 | 0 |

9 4 |

Regions are numbered from left to right. In the columns, each pair of classes represents progeny of the two complementary classes. The top number is for the class having the allele of the leftmost locus that is uppermost in the zygote genotype diagram. For example, in the first cross, there were 14

*cya-8*progeny, 6^{+}T*cya-8 N*, 0*cya-8*, and 1^{+}N*cya-8 T*. See Perkins*et al*. (2001) for information on markers. The*cya-8*locus is included in the segment translocated to VR in translocation*ALS179*, but*cyt-7*and other VIIL markers are not included (Perkins 1997).*adh*, adherent morphology;*cya*-*8*, cytochrome*a*defective;*cyt-7*, cytochromes*a*and*b*defective;*nic-3*, nicotinamide requirement;*N*, normal chromosome sequence;*T*, quasi-terminal translocation*T(VIIL*→*VR)ALS179*.↵

*a*The ratio of phenotypically cyt^{+}:cyt^{−}was 21:44, as expected if the*cyt-7*locus is not included in the translocated segment, which is duplicated in one-third of viable meiotic products. Only 17 of the 44 cyt^{−}progeny were scored for Barren,*T*, and*N*. Of these, 11 were Barren duplications and 6 were fertile normal sequence, confirming that*cyt-7*is right of the*T(ALS179)*breakpoint in VIIL. The order is thus*cya-8 T cyt-7 adh nic-3*.