TABLE 1

Microsatellite markers typed in HG2DF2 and HG11F2 mice

MarkerMMUPCR conditionscMMbpGenotype counts (C/H/B/missing)χ2
D2Mit329255/2.00.074.971/133/74/00.75
D2Mit93255/1.50.076.769/131/73/50.76
D2Mit94255/1.51.780.072/130/76/00.53
D2Mit160255/1.53.084.869/133/76/00.65
D2Mit439255/2.05.092.069/133/75/10.71
D2Mit130255/1.55.897.469/134/75/00.73
D2Mit389255/1.57.4103.168/133/77/00.58
D2Mit207255/1.59.1112.069/130/79/00.39
D2Mit17255/1.513.2122.663/127/71/170.71
D2Mit224255/2.016.5129.065/139/74/00.75
D2Mit223255/2.019.7134.763/146/69/00.62
D2Mit212255/1.523.3141.768/145/65/00.75
D2Mit260255/1.526.3148.966/147/65/00.63
D2Mit262255/2.030.1155.666/141/71/00.89
D2Mit196255/2.033.5160.262/147/69/00.53
D2Mit454255/2.036.8164.057/150/70/10.21
D2Mit456255/1.541.4168.756/155/64/30.09
D2Mit213255/2.050.1174.361/145/71/10.51
D2Mit148255/1.551.5179.357/147/73/10.24
D11Mit2611155/2.00.062.293/243/114/40.09
D11Mit51155/1.51.267.392/244/116/20.07
D11Mit901155/2.02.970.695/237/120/20.15
D11Mit2801155/2.06.077.195/240/117/20.14
D11Mit361155/1.59.083.993/242/117/20.09
D11Mit2851158/2.013.490.092/245/112/50.06
D11Mit671158/1.517.297.191/245/116/20.05
D11Mit3301155/2.019.299.691/248/114/10.04
D11Mit101155/2.022.6104.793/245/113/30.08
D11Mit2241155/2.025.5108.698/239/109/80.24
D11Mit2551155/3.033.8114.3110/213/114/170.84
  • PCR conditions, annealing temperature/MgCl2 concentration; cM, relative genetic position of each marker within each congenic donor region calculated using cross data; Mbp, genomic position according to the August 2005 mm7 UCSC genome assembly (NCBI Build 35); genotype counts, number of mice with C (CAST/CAST), H (HG/CAST), B (HG/HG), or missing genotype at each marker; χ2, P-value for χ2 test of Mendelian segregation. Markers did not significantly deviate from Mendelian segregation after Bonferroni correction for multiple comparisons.