TABLE 6

Tetrad analysis of spo11-HA diploids

GenotypeaHIS4–LEU2 PD:NPD:TTbcMc (foldd)LEU2–MAT PD:NPD:TTbcMc (foldd)MAT–iADE PD:NPD:TTbcMc (foldd)ARG4–THR1 PD:NPD:TTbcMc (foldd)
Wild type218:7:12323.7 (1.0)180:5:16527.9 (1.0)123:12:22841.3 (1.0)298:0:375.5 (1.0)
sgs1795275:19:18731.3e (1.3)193:19:26639.7e (1.4)179:34:30949.1 (1.2)428:0:606.1 (1.1)
spo11-HA379:6:25522.7 (1.0)276:10:36732.7 (1.2)128:23:27848.5 (1.2)584:0:655.0 (0.9)
sgs1795 spo11-HA392:1:16015.0e (0.6)318:11:22426.2 (0.9)251:24:32439.1 (0.9)515:0:615.3 (1.0)
  • a Strains analyzed were BR4337, BR4474, BR4357, and BR4475.

  • b PD, parental ditype; NPD, nonparental ditype; TT, tetratype.

  • c Map distances were calculated from four-spore viable tetrads using Perkin's formula (Perkins 1949).

  • d The fold increase in map distance relative to wild type is indicated.

  • e Map distances are significantly different from those in wild type.