TABLE 1

Insertion sites and candidate genes identified in the screen

P-GS line (no. insertions)Citologyshv-Gal4D5′ geneMolecular classNo. candidatesD3′ geneMolecular class
C649 (1)1E3B0
EP-63 (1)3A1V−3CG14049 (Ilp6)CS27CG2845 (phl)CS
EP-489 (1)3C6V+11CG3653 (kirre)CS, CA
EP-A (2)3C7V+10CG3936 (N)CS
C155 (3)4B2V+1CG3665 (Fas2)CA1
EP-B5 (1)4C4F10CG3578 (omb)TF
EP-620 (4)4E2CD0CG32767TF1
EP-469.2 (4)5B5V−1CG3171 (Tre1)CS21CG15779 (Tre)CS
C684 (1)7C4B0CG10778M21CG1524 (RpS14a)RB
C858 (1)8B4V+0CG10701 (Moe)CY1
C76 (1)9D2V+2CG15302 (or9a)CS20CG15304CG
EP-165 (6)9E1V+1CG32676PP20CG1799 (ras)M
C719.2 (1)9F5CD1CG1655CS20CG2186CG
EP-1052 (1)9F5Dif0CG11207 (feo)CY24CG2186CG
EP-Z (1)10C9V+0CG1697 (rho-4)CS1
EP-694 (1)11A11Pol1CG1900 (Rab40)CS21CG17788CG
EP-332 (1)11D1F6CG33651CG1NO
EP-689 (1)12E5CDNO10CG12047 (mud)CGh
EP-457 (5)12F4B0CG9533 (rut)M27CG14411M
C373 (3)13F1BNO11CG8544 (sd)TF
C736 (2)13F17V−0CG9056CG1
C375 (1)15C4CD1CG9089 (wus)PP1NO
EP-493 (1)15F4B3CG8915 (helicasa)RB22CG12996CG
C832 (1)16C1B0CG32556CG1
EP-631 (1)16C1CD1CG32556CG24CG5884 (par-6)CA
C800 (1)17A8B10CG6103 (CrebB-17A)TF
EP-471 (1)21B2V+NO10CG18497 (spen)TF
EP-234 (1)21B4V−2CG11617TF1
C31 (1)21C2V+0CG11907 (Ent1)M1
C580 (1)21C4wtNO10CG4114 (ex)CS
EP-67 (2)21C6Pol0
C480 (4)21C6CD0CG4427 (cabut)TF1
EP-822 (1)21D1V+1CG17941 (ds)CS, CA1
EP-720 (2)22C1V+2CG15378 (lectin-22C)M20CG4244 (su(dx))PP
C676 (2)22E1V+0CG3664 (Rab5)CS20CG4272CS, CD
C517 (3)22F2V+16CG9885 (dpp)CS
EP-500.2 (1)24A1B0CG10033 (for)CS1
C37 (2)24C3BNO10CG10021 (bowl)TF
C544 (1)25B1BNO11CG3036M
EP-32225B10F0CG33113 (Rtnl1)CS1
EP-800 (1)25B3F0CG8890 (Gmd)CS1
C575 (1)25B9F1CG8892PP27CG31653CG
EP-787 (1)25C1B8CG8680M1
EP-472 (1)26A1Dif2CG9021CG20CG14001 (bchs)PP
C174 (3)26A5CD0CG9553 (chic)CY22CG9075 (eIF-4a)RB
C386 (2)26B2CD0CG9088 (lid)TF21CG9093 (Tsp26A)CS
C446 (4)26B3F1CG9154CGh20CG9159 (Kr-h2)TF
EP-603 (2)27F1DifNO13CG5261M
EP-M71 (1)27F3CD10CG4971 (Wnt10)CS
EP-60028D1V+10CG7123 (LanB1)CA
EP-899 (2)28D3V−1CG7233 (snoN)CS, TF1
EP-24 (4)29A1V+0CG8049 (Btk29A)CS1
EP-232 (1)29C3V+1CG13398CS21CG13388 (Akap200)CS
C503 (3)29C3F4CG13398CS20CG13388 (Akap200)CS
C717 (2)30B5V+, CD0CG4405 (jp)CY22CG3838Cgd
C388 (4)30B8CD1CG4422 (Gdi)CS1
C282 (1)30C7B10CG3998 (zf30c)TF
EP-108 (1)30F4V+1CG5838 (Dref)TF20CG4651 (RpL13)RB
C488 (1)30F5V+11CG4722 (bib)CS
EP-112 (2)31A1V−NO10CG4799 (Pen)M
EP-731 (1)31E1Dif0CG5355PP21CG5300 (Klp31E)CY
C242 (1)32B1F0CG6647 (porin)M21CG17085CG
EP-179 (1)32E1B0CG4807 (ab)TF1
EP-(19) (1)32E2V−1CG6392 (cmet)CY20CG32955 (Cana)CY
EP-476 (1)32F2Dif0CG31705CG1NO
EP-Ib (6)33A1B0CG14938 (crol)TF1NO
EP-M32 (2)33B3V+1CR31863 (bft)snRNA1
C762 (2)33F4V−1CG12283 (kek-1)CS1
EP-1008 (2)34D4V+11CG7793 (Sos)CS
C891 (2)35A3B1CG4551 (smi35A)CS1
C143 (16)35D2CD0CG3758 (esg)TF1
EP-339 (5)35E2V−0CG4993 (PrL-1)CS27CG4930CG
C17 (5)35F1V−0CG7664 (crp)TF21CG4132 (pkaap)CS
EP-64 (1)36A2B1CG5953CG1
C877 (1)36A2V+8CG4952 (dac)TF20CG4599 (Trp2)PP
EP-M (2)36C5V+0CG6667 (dl)TF23CG5050CG
EP-323 (2)37B11V−1CG15173CGd20CG10473CD
C623 (1)37D7CD0CG10334 (spi)CS1
C719.1 (1)38E3B1CG9318M20CG2637 (Fs(2)Ket)M
C359 (1)38F1BNO10CCG31673M
C277 (6)39B4V+, CD1CG31626CG20CG8676 (Hr39)TF
EP-319 (2)42A4CD0CG12051 (Act42A)CY20CG7865 (PNGase)PP
C504 (2)42E1F1/4CG3572 (vimar)/CG30156CS/PP30CG17002CG
C109 (1)42E4F7CG3572 (vimar)CS30/0CG18742 (Tsp42Ea)/CG30159CS
C573 (1)42E5F1CG12846 (Tsp42Ed)CS20CG10106 (Tsp42Ee)CS
C371 (2)43B1B1CG1708 (cos)CY21CG11107RB
EP-3 (3)44C2V−1CG3161 (dpn)TF1
C434.2 (1)44C4V+, CDNO0
EP-405 (1)44E3CD0CG8739 (cmp44E)CA23CG8740CG
C935 (1)44F7CD0CG8248CY21CG8243CS
EP-M89 (1)45C3V−1CG2072 (TXBP181-like)CGh20CG1975 (Rep2)CGd
EP-65 (1)45F1V−7CG1888CG21CR33010 (mir-14)microRNA
EP-23 (6)46C4V−0CG1429 (Mef2)TF1
C356 (1)46D7V+1CG18445CGh20CG2249M
EP-87847A1B0CG3298 (JHI-1)RB20CG12909RB
C839 (1)47C1CD1CG12342CGd20CG12323 (Pros b5)PP
C192 (4)47D7B0CG7734 (shn)CS, TF1
C170 (3)48A2F0CG10897 (tou)TF1NO
C7 (1)49A4V+, CD4CG8834M20CG8525M
C478 (1)50C14V+0CG6671 (AGO1)RB30/0CG33155/CG30481 (mRpL53)CG/M
EP-990 (3)50D1V+10CG8118 (mam)CS
C56 (4)50E6F1CG8542 (Hsc70-5)M20CG8531M
EP-608 (2)51E3F10CG11798 (chn)TF
C603 (1)51F11CD1CG8171 (dup)TF20CG8174 (SRPK)CS
C207 (1)52A8F0CG8183 (Khc-73)CY25CG30471CG
EP-M21 (1)52D9wt0CG8322 (ATPCL)M22CG8370CGh
C27 (2)52F8B1CG8448 (mrj)M1NO
C231 (5)53D12wtNO13CR33562 (mir8S)microRNA
C79 (1)53F1CDNO10CG9635 (RhoGEF2)CS
C459 (12)53F9V+1CG8938 (GstS1)M26CG30456Cgh
EP-887 (1)54B16wt0CG6522CY21CG4816 (qkr54B)RB, CD
EP-687 (1)54F1CD3olf186-MPP20CG11430 (olf186-F)PP
C367 (4)55B7CD4CG5753 (stau)RB20CG12767 (Dip3)TF
EP-J59 (1)55B8Dif7CG5748 (Hsf)TF20CG5119 (pAbp)RB
EP-773 (1)55F6V+, CD1CG15098CG20CG15083CG
C677 (1)56A1B10CG12758 (sano)CG
C639.1 (2)56C1V+, CDNO11CG7097CS
C518 (4)56E3V−, CD2CG9854 (hrg)RB22CG11025 (IsoT-3)PP
EP-614 (3)56F16B4CG13868CG20CG8920RB
EP-709 (1)56F8V+11CG8896 (18w)CS
C389 (2)57A7CD0CR33617 (mir-313)microRNA24CG13425 (bl)RB
EP-947 (1)57C4Dif0CG30389CG1
C596 (1)57E5B0CG10497 (Sdc)CS27CG15667 (sara)CS
C327.2 (1)57E5V+0CG9847 (Fkbp13)PP25CG15669 (Mesk2)CGd
EP-634.2 (1)57E8CD8CG10496CG20CG15669 (Mesk2)CGd
EP-439 (1)57F10V−1CG30404CGh21CG17952CA
EP-296 (1)58D3wt7CG3413 (wdp)CA21CG5820 (Gp150)CS
EP-575 (1)58E4V+0CG4444 (px)TF1
EP-596 (1)58F4Dif2CG30217CG23CG4250CG
EP-1152.259B6V−, E1CG3820 (Nup214)M1
EP-O (1)59F6wt10CG5393 (apt)TF
C125 (1)60C7CD1CG4527 (Plkk1)CS21CG3416 (Mov34 )PP
EP-708 (1)60D9V−0CG13594CG21CG3616 (Cyp9c1)M
EP-261 (1)60E1wt0CG16912RB21CG3589PP
EP-284 (1)60E1V+1CG16932 (Eps15)M25CG3594 (Eap)RB
EP-644 (2)60E5V+10CG9071 (NaCP60E)M
EP-945 (1)61B3wt1CG17135 (E(bx))TF21CG32476 (mthl14)CG
C293 (1)61B3CD1CG7008 (Tudor-SN)TF21CG13880 (mRpL17)RB
EP-880 (2)61C3V+1CG13892 (Cypl)PP22CG17090CS
EP-429 (1)61F6B2CG2211CG20CG9165M
C865 (4)61F7V+0CG9181 (Ptp61F)CS24CG1214 (ru)CS
C767 (1)62A1V+1CG13916TF1
EP-450 (1)62A3CD0CG12086 (cue)M20CG1009 (Psa)PP
C500 (9)63C1V−5CG12078CGh22CR33598 (mir-282)microRNA
EP-329 (1)64A7B0CG14995CD21CG14991CA
C549 (1)64D1F0
C275 (2)64E5CD1CG10578 (DnaJ-1)PP20CG5486 (Ubp64E)PP
EP-1 (1)65C3V+0CG10107PP26CG8549CGh
C38 (1)66A13F0CG17888 (Pdp1)TF1
C255 (1)66B3CD6/4CG7574 (bip1)/CR32358CG/tRNA38CG13681CG
C545 (1)66C11Dif0CG7163 (mkg-p)TF21CG13667M
C107 (3)66D9V−10CG6494 (hairy)TF
C403 (1)67B5B0CG3445 (phol )TF23CG3552CG
C432 (1)67C4CD1CG6757 (SH3PX1)PP20CG16707 (vsg)CGd
C708 (1)67C7V+3CG6767M20CG8284 (UbcD4)PP
EP-730 (2)67C9V+0CG6721 (Gap1)CS1NO
EP-380 (1)67D2Pol1CG6674CG20CG11989 (Ard1)PP
C67 (1)67F4B0CG12296 (klu)TF1
C756 (1)68B1B0CG6190PP21CG7600CGh
EP-297.2 (1)68F1V+1CG12277 (rols)CS1NO
C909 (1)69A4V+0CG4300M21CG10426M
EP-154 (1)69D3wt0CG10601 (mirr)TF1
EP-872 (1)69F4FNO10CG11278 (Syntaxin13)PP
EP-J17b70B1B0CG10133M1NO
EP-853 (1)70B2wt1CG10083CY21CG10741CG
EP-1013 (1)70E5V−1CG4879 (RecQ5)TF20CG5031 (dlp)CS
EP-501 (1)72D6V−2CG5215 (Zn72D)RB20CG5444 (Taf4)TF
C747 (1)72F1V−0CG4531 (argos)CS1NO
C427 (1)73D1V+, CD3CG9668 (Rh4)CS20CG11914 (Lmpt)TF
EP-666 (1)73D1F10CG9712 (TSG101)PP
C153 (2)74D2F4CG6311CGh20CG7555 (Nedd4)PP
EP-182 (1)75B1B10CG8127 (Eip7B)TF
C272 (1)75D1B1CG13702 (AlCR2)CS1
EP-160 (3)75F6V−1CG14080 (Mkp3)CS1
EP-P (2)76D1B0CG8742 (Gyc76C)CS23CG8522 (HlH106)TF
C752 (1)76D3B0/1CG8103 (Mi-2)/CG32217PP30CG7757RB
C474.1 (2)77B4V+1CG5585CGh20CG5605 (eRF1)RB
C167 (1)78B1B4CG10564 (Ac78C)CS20CG32435 (chb)CY
EP-17 (3)83A5V−0CG2899 (ksr)CS22CG31550TF
EP-256 (4)85C1V−1CG11988 (neur)CS1
C606 (2)85C4CD0CG9755 (Pum)RB1
C926 (1)85D1V−2CG9746CS1
C343 (1)85D21V+1CG9375 (Ras85D)CS20CG8161 (Rlb1)CGd
EP-643 (2)85D22V+0CG9381 (mura)CGd21CG16788 (RnpS1)RB
C436 (2)85D25CD0CG9399CGh24CG8273RB
EP-686 (2)85E14F11CG12418CG
C441 (1)86F7CD1CG17342 (LK6)CS1NO
C547 (1)86F7Bs1CG31364 (l(3)neo38)CG28CG14723 (HisCl1)CS
EP-650 (2)87D9V+1CG12360CGh20CG7620 (l(3)87Df)CGh
C18 (4)87D9CD2CG8031CGh20CG7583 (CtBP)TF
EP-1152.1 (2)87E11V+0CG9764 (yrt)CY1
EP-149 (4)88A4B0CG9924PP20CG3050 (Cyp6d5)M
C840 (1)88C6V+0CG7832CG1
EP-A7 (1)88D1bx0CG7530CGh1NO
EP-536 (1)88D2V+0CG7425 (eff)PP21CG3563CG
C875 (1)88E1B0NO
EP-J76 (1)88E3CD0CG6535 (tefu)CS20CG4264 (Hsc70-4)PP
C278 (7)88E4V+0CG6499M23CG4285CG
EP-158 (1)88F1V−1CG6202 (Surf4)CS20CG31301TF
EP-767.1 (1)89A8V+1CG18740 (mor)TF24CG4261 (Hel89B)TF
C618 (10)89B12V+10CG6889 (tara)TF
EP-207 (4)89B12V+, CD10CG6963 (gish)CS
EP-167 (2)89C7V+0CG14895 (Pak3)CS1
C607 (1)89D5F1CG14905CGh20CG6588 (Fas1)CA
EP-435 (2)89E11V−10CG5201 (Dad)CS
EP-87 (1)89F3V+0NO
C923 (1)90A3B1CG31256 (Brf)TF20CG5851 (sds22)PPh
C819 (6)90C1V+0CG7467 (osa)TF1
EP-36 (4)92A1V+1CG3619 (Dl)CS1
C904.1 (3)92B2F0CG4608 (bnl)CS24CG31459CG
C246 (1)92C1B0CG4413TF21CG4936TF
C861 (1)92F1V+2CG4241 (att-ORFA)M21CG5180CG
EP-M68 (2)92F1V+10CG5460 (H)CS, TF
EP-55 (4)92F1V+0CG4257 (Stat92E)CS, TF1
C407 (3)93B2V+10CG5670 (Atpalpha)M
EP-572 (1)93B3V−1CG3593 (r-l)M22CG5737 (dmrt93B)TF
C325 (1)93C7B1CG3337CGh20CG5874TF, RB
C211 (1)93E9F0CG6376 (E2f)TF1
EP-J63.2 (1)94E1V−0CG17894 (cnc)TF1
C279 (4)94E1V−1CG4637 (hh)CS1
C411 (1)94E9CD0CG4449PP21CG6755TF, RB
EP-820 (1)94E9V+4CG13825CG23CG6755TF, RB
C588 (2)94E9wt0CG10868 (orb)RB21CG6759 (CDC16)PP
EP-111 (2)94E9V−2CG17077 (pnt)CS, TF22CG6768 (DNApol-epsilon)M
EP-654a (1)95A7DifNO10CG10161 (eIF-3p66)RB
C933.1 (1)95B1V+8/9CG33111/CG10192RB31CR31185 (snRNA:U1:95Cc)snRNA
C919 (1)95C5F0CG5320 (Gdh)M1NO
C790 (1)95D7B0/0CG5448 (Syx1A)/CG33110M/CG32CG10694CGh
EP-51 (1)96D4wt0CG4548 (XNP)TF21CG5116CS
EP-469.1 (1)96F10V−7CG6096 (m5)TF21CG8361 (m7)TF
C442 (7)98A2V−0CG5643 (wdb)PPh21CG5692 (raps)CS
EP-298 (2)98F1B3CG33203CG20CG1658 (Doa)CS
C495 (1)99D1V−1CG15525CGh20CG11504CGd
C328 (1)99E4wt2CG2216 (Fer1HCH)M20CG1469 (Fer2LCH)M
EP-610 (1)100B1B0CG1715 (l(3)03670)CGh1NO
C166 (2)100C7CD0CG11525 (CycG)CS20CG1775 (Med)CS, TF
C345 (1)100D2CD1CG2126CG21CG1945 (faf)PP
C632 (2)100D2F1CG2210 (awd)M21CG1910CG
C705 (1)102A3F10CG17964 (pan)TF
EP-241 (1)102C1B0CG11533CS1
EP-140 (1)102D1CD3CG11091 (sphinx)CG1
  • Each insertion site is represented by one P-GS line. Data are sorted by cytology. Phenotype in combination with Gal4-shv3Kpn (shv-Gal4): thicker veins (V+), loss of veins (V−), defects in cell differentiation (CD), fails in dorso–ventral adhesion (B), defects in wing folding (F), alterations in polarity (Pol), defects in epithelial integrity (E), and wild type (wt). Molecular class: cell signaling (CS), transcription factor (TF), cell adhesion (CA), cytoskeleton (CY), metabolism (M), proteases (PP), RNA binding (RB), annotated genes with unknown function without homology (CG), those with homology (CGh), those with conserved structural domains (CGd), microRNA, snRNA, and tRNA. D, distance in kilobases of transcription initiation to the insertion site.