TABLE 5

Characteristics of the well-studied mutant alleles of rec10

rec10 alleleMutationDSB proficient?Crossover proficiencyGene conversion proficiencyade6–M26 hot spot proficiencyLinE statusOther
rec10-175aFull open reading frame deletionNoNonebNonebNonebNonec
rec10-109dTwo point mutationsa G526A (V176I) and G533A (G178D)eLevels of DSBs diminishedfRegionalghRegionalghPartialgAbnormal LinEs formedciComplements rec10-144jk. Slightly temperature sensitive for recombination at some regionsa
rec10-144jkSingle point mutationj G2180A (G727E)eNot determinedLimited reduction at all intervals testedlLimited reduction at all intervals testedjlReduced hot spot activityjLimited abnormal LinEs formedjmComplements rec10-109jk
rec10-155nInsertion inactivation mutant; presumed C-terminal truncation of last 103 amino acidsnNot determinedLimited reduction at all intervals testedlLimited reduction at all intervals testedlnReduced hot spot activityoNoneimp
  • Other rec10 mutant alleles have been isolated/generated and sequenced (De Veaux et al. 1992; Ellermeier and Smith 2005; our unpublished data), but they have undergone only limited analysis to date and are not listed here.

  • a Ellermeier and Smith (2005).

  • b None equates to the levels observed for a rec12 null mutant.

  • c As measured by immunocytochemistry.

  • d Ponticelli and Smith (1989).

  • e Numbered from the start of the open reading frame.

  • f Cervantes et al. (2000).

  • g De Veaux and Smith (1994).

  • h Krawchuk et al. (1999).

  • i Lorenz et al. (2004).

  • j Pryce et al. (2005).

  • k De Veaux et al. (1992).

  • l This study.

  • m As determined by immunocytochemistry and electron microscopy.

  • n Lin and Smith (1995).

  • o D. W. Pryce and R. J. Mc Farlane (unpublished observation).

  • p Molnar et al. (2003).