TABLE 2

Intragenic gene conversion frequencies for two-factor rec10-155 and rec10-144 homozygous crosses

rec10 allelePlasmidLocusMean recombination frequencyaFold reductionb
rec10+ade6c297 ± 114
rec10-155ade61.2 ± 0.3247
rec10+lys7d14 ± 10
rec10-144lys70.3 ± 0.247
rec10-155lys70.4 ± 0.435
rec10+ura1e212 ± 51
rec10-155ura135 ± 56
rec10-175ura13.0 ± 0.570f
rec10-175pSP1gura11.8 ± 1.2118
rec10-175pYL167gura124 ± 109
  • a Number of prototrophs per 106 viable spores ± 95% confidence interval; n ≥ 4 in all cases.

  • b Student's t-test P-values from pairwise comparisons of rec10+ vs. rec10 gave values of P ≤ 0.01 in all cases.

  • c Alleles used: ade6-M375 × ade6-52.

  • d Alleles used: lys7-1 × lys7-2.

  • e Alleles used: ura1-61 × ura1-171.

  • f This was the lowest fold reduction obtained in this study for ura1 intragenic recombination. Other mean data values resulted in fold reductions as high as 155-fold.

  • g pYL167 is the plasmid carrying the rec10-155 allele (Lin and Smith 1995). pSP1 is a vector control containing no rec10 cloned DNA.