TABLE 1

Oligonucleotides generated for this study

NameSequence 5′–3′Description
JG94TCAAAATGTCAGTTTATGCAGTTTTCACGGTATTGTTCTCGATAATTTTTGGTGACGGTGCTGGTTTAAmplifies GFP:kanMX4 and targets to SPS2
JG95TATCTCATAAGAACTAAATAAAATGGCTAGTATTTAAGTTAATCTTCTAACTCGATGAATTCGAGCTCGAmplifies GFP:kanMX4 and targets to SPS2
BC336GGATACAGTTCTCACATCACATCCGAACATAAACAACCATGGComplementary to MX4 promoter
BC337CGTTAGTATCGAATCGACAGCAGTATAGCGACCComplementary to MX4 terminator
JG111GTGGGAATAACAAGCGCGCTACTGGTAAGTGAmplification of RME1 QTL
JG117CGGTTGCACCTCTTGTAAAACCTCTCTGTTCCAmplification of RME1 QTL
JG120CGTAATAACCATTGAACTACATTAAAGAAATAACTGTCTCGCAmplification of MKT1 QTL
JG124GGCATTGGTACCGCTGCTGGAmplification of MKT1 QTL
JG128CCCAAACATTGGACGATGATTTAGAGTTGCCAmplification of TAO3 QTL
JG133CCCTTGCCAAGAGAAATCCATAGCTGCAmplification of TAO3 QTL
JG113GGGATAGTGGGTAAACGAGTAGCSequencing of RME1 QTL
JG114GCTTCGTCGTTGGTATAGTTTCGGSequencing of RME1 QTL
JG119GTCCATTAACTATTTTAGCGTGACTGACSequencing of MKT1 QTL
JG123GCTGCAGTAAAATGGCCCGSequencing of MKT1 QTL
JG127CCTTCGGTAGCTGTTTTATATTACACTACSequencing of TAO3 QTL
JG130CGAAAGGAAACAAATCAAGTATTCTAACCSequencing of TAO3 QTL