TABLE 2

Efficiency of RNAi experiments determined by RT–qPCR

StrainmRNA assayedTranscript fold decreasea
daf-2(e1370);gpd-2/3(RNAi)gpd-2/39.67
daf-2(e1370);gpi-1(RNAi)gpi-116.40
daf-2(e1370);enol-1(RNAi)enol-112.91
daf-2(e1370);pgk-1(RNAi)pgk-17.91
  • For all RNAi experiments, the level of transcript was significantly reduced in comparison to the control strain (P < 0.05, as determined by the Mann–Whitney U-test).

  • a Transcript level was determined relative to the daf-2(e1370) control strain.