Phenotypes assessed in the project
| Test | Measure |
|---|---|
| Open field arena | Total activity |
| Fecal boli | |
| Elevated plus maze | Closed-arm distance |
| Open-arm distance | |
| Closed-arm time | |
| Open-arm time | |
| Closed-arm entries | |
| Open-arm entries | |
| New home-cage activity | Total beam breaks (30 min) |
| Total beam breaks (first 5 min) | |
| Total beam breaks (last 5 min) | |
| Fine movement | |
| Context freezing | Time freezing to context (sec) |
| Cue conditioning | Time freezing during cue (sec) |
| Time freezing after cue (sec) | |
| Fecal boli | |
| Fear-potentiated startle | Startle response |
| Change in startle after training | |
| Plethysmography | Enhanced pause (baseline) |
| Enhanced pause (metacholine) | |
| Expiratory time (baseline) | |
| Expiratory time (metacholine) | |
| Inspiratory time (baseline) | |
| Inspiratory time (metacholine) | |
| PenH difference | |
| Respiratory rate (baseline) | |
| Respiratory rate (metacholine) | |
| Tidal minute volume (baseline) | |
| Tidal minute volume (metacholine) | |
| Tidal volume (baseline) | |
| Tidal volume (metacholine) | |
| IPGTT | AUC-G (mg/dl) |
| AUC-IRI (ng/ml) | |
| AUC-IRI/AUC-G | |
| DG (mg/dl) | |
| DIRI (ng/ml) | |
| DIRI/DG | |
| Glucose 0 (mg/dl) | |
| Glucose 15 (mg/dl) | |
| Glucose 30 (mg/dl) | |
| Glucose 75 (mg/dl) | |
| Insulin 0 (ng/ml) | |
| Insulin 15 (ng/ml) | |
| Insulin 30 (ng/ml) | |
| Insulin 75 (ng/ml) | |
| Insulin slope | |
| K (glucose slope) | |
| Immunology | %B220+ |
| %CD3+ | |
| %CD4+ | |
| %CD4+/CD3+ | |
| %CD8+ | |
| %CD8+/CD3+ | |
| %NK cells | |
| Hematology | Hematocrit (%) |
| Hemoglobin (g/dl) | |
| Mean cellular volume (fl) | |
| Platelets (n/μl) | |
| Red blood cell count (n/μl) | |
| White blood cell count (n/μl) | |
| Mean cellular Hb concentration (%) | |
| Red cell distribution width | |
| Mean corpuscular hemoglobin (pg) | |
| Lymphocytes | |
| Plateletcrit (%) | |
| Biochemistry | Alkaline phosphatase (units/liter) |
| Alanine transaminase (units/liter) | |
| Aspartate transaminase (units/liter) | |
| Albumin (g/liter) | |
| Calcium (mmol) | |
| Chloride (mmol) | |
| High-density lipoproteins (mmol) | |
| Low-density lipoproteins (mmol) | |
| Phosphorous (mmol) | |
| Sodium (mmol) | |
| Total cholesterol (mmol) | |
| Total protein (g/liter) | |
| Triglycerides (mmol) | |
| Urea (mmol) | |
| Weight, length, and growth | Body length |
| Body mass index | |
| Growth slope | |
| Weight, 10 wk | |
| Weight, 6 wk | |
| Weight, 7 wk | |
| Weight, 8 wk | |
| Adrenal weight | Adrenal weight (g) |
| Wound healing | Ear hole area (from ear punch) (mm2) |