Summary of phenotypes analyzed, number of animals, and mean age (in days) at which the animals were analyzed
| Phenotype | Description | No. of animals | Mean age (days) |
|---|---|---|---|
| Weight, 6 wk | Body weight at the beginning of testing. | 2516 | 42 |
| Immunology | CD4, CD3, CD8, and B220 antibody staining. | 1872 | 42 |
| OFT | Open field arena: distance in the perimeter, the center, and total distance in 5 min. | 2504 | 45 |
| EPM | Elevated plus maze: distance traveled, time spent, and entries into closed and open arms. | 2452 | 46 |
| FN | Food hyponeophagia: time taken to sample a novel foodstuff (overnight food deprivation). | 2474 | 47 |
| Burrowing | No. of pellets removed from burrow in 1.5 hr. | 2455 | 48 |
| Activity | Activity measured in a home cage in 30 min. | 2445 | 48 |
| Startle | Startle to a loud noise. | 1948 | 52 |
| Context freezing | Freezing to the context in which a tone is associated with a foot shock. | 2070 | 55 |
| Cue freezing | Freezing to a tone after association with a foot shock. | 2110 | 56 |
| Plethysmography | Animals sensitized by injection with ovalbumin inhale metacholine and changes in lung function are measured by plethysmography (a model of asthma). Respiratory rate, tidal volume, minute volume, expiratory time, inspiratory time, and enhanced pause are recorded with and without exposure to metacholine. | 2304 | 63 |
| IPGTT | Glucose and insulin values taken at 0, 15, 30, and 75 min after i.p. glucose injection (a model of type 2 diabetes mellitus). | 2334 | 68 |
| Weight, 10 wk | Body weight at the end of testing. | 2319 | 70 |
| FBC | Full blood count (hematocrit, Hb concentration, mean cellular volume, mean cellular Hb concentration, white cell count, platelet count). | 1892 | 71 |
| Tissue harvest | Adrenal weight. | 2309 | 71 |
| Wound healing | Reduction in size of a 2-mm ear punch hole. | 2273 | 71 |
| Biochemistry | Albumin, alkaline phosphatase, alanine transaminase, aspartate transaminase, calcium, chloride, creatinine, high-density lipoprotein, low-density lipoprotein, phosphorous, sodium, total cholesterol, total protein, triglycerides. | 1890 | 71 |