TABLE 3

Distribution of the actin cytoskeleton at the mother-bud neck of cells with elongated buds

Actin localization at the mother-bud neck of cells with elongated buds
Protein overproducedNoneNormalDiffused
HA-Skg6281062
HA-Tos273720
  • Haploid wild-type (CCY1710-3C) cells and cells overproducing Skg6 (CCY1708-1A) or Tos2 (CCY1709-2B) were cultured at 26° in YEP-raffinose (2%) medium to log phase. Galactose (4%) was then added to the growth medium to induce overproduction of HA-Skg6 and HA-Tos2 and cultures were incubated further at 37° for 6 hr. Cells were fixed with formaldehyde (3.7%), stained with rhodamine–phalloidin, and examined by fluorescent microscopy. None of the wild-type cells formed elongated buds and therefore are not listed.