Morphological analysis of cells overproducing Skg6 or Tos2
| Budded (%) | ||||||
|---|---|---|---|---|---|---|
| Growth condition | Protein overproduced | Unbudded (%) | Single regular bud | Single elongated bud | Two separate buds | “Bud from a bud” |
| 26° | — | 46 | 54 | 0 | 0 | 0 |
| YEP-raffinose | HA-Skg6 | 40 | 60 | 0 | 0 | 0 |
| HA-Tos2 | 45 | 55 | 0 | 0 | 0 | |
| 26° | — | 49 | 51 | 0 | 0 | 0 |
| YEP-galactose for 6 hr | HA-Skg6 | 41 | 56 | 3 | 0 | 0 |
| HA-Tos2 | 50 | 48 | 2 | 0 | 0 | |
| 37° | — | 46 | 51 | 0 | 3 | 0 |
| YEP-galactose for 6 hr | HA-Skg6 | 34 | 14 | 27 | 6 | 19 |
| HA-Tos2 | 46 | 38 | 10 | 3 | 3 | |