TABLE 2

Morphological analysis of cells overproducing Skg6 or Tos2

Budded (%)
Growth conditionProtein overproducedUnbudded (%)Single regular budSingle elongated budTwo separate buds“Bud from a bud”
26°4654000
YEP-raffinoseHA-Skg64060000
HA-Tos24555000
26°4951000
YEP-galactose for 6 hrHA-Skg64156300
HA-Tos25048200
37°4651030
YEP-galactose for 6 hrHA-Skg6341427619
HA-Tos246381033