TABLE 1

Yeast strains used in this study

StrainGenotype
CCY1024-19Cαhis3-Δ200 leu2-3,112 trp1-1 ura3-52 gic1-Δ1∷LEU2 gic2-1∷HIS3
CCY1137-3Baade2 his3-Δ200 leu2-3,112 ura3-52 trp1-1 hof1-Δ101∷spHIS5 with pCC1368 (CEN, URA3, HOF1)
CCY1176-11Aαlys2-801 his3-Δ200 leu2-3,112 ura3-52 trp1-1 skg6-Δ1∷kan tos2-Δ2∷URA3
CCY1244-4Dαhis3-Δ200 leu2-3,112 trp1-1 ura3-52 hof1-Δ101∷spHIS5
CCY1244-7Cαhis3-Δ200 leu2-3,112 trp1-1 ura3-52 hof1-Δ101∷spHIS5 skg6-Δ1∷kan
CCY1244-10Dαhis3-Δ200 leu2-3,112 trp1-1 ura3-52 hof1-Δ101∷spHIS5 tos2-Δ2∷URA3
CCY1244-17Cαhis3-Δ200 leu2-3,112 trp1-1 ura3-52 hof1-Δ101∷spHIS5 skg6-Δ1∷kan tos2-Δ2∷URA3
CCY1292-10Cαlys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 vhs2-Δ1∷TRP1 mlf3-Δ1∷kan
CCY1292-1Aalys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52
CCY1292-1Dalys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 mlf3-Δ1∷kan
CCY1292-4Balys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 vhs2-Δ1∷TRP1
CCY1292-5Balys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 vhs2-Δ1∷TRP1 mlf3-Δ1∷kan
CCY1338aade2/+ lys2-801/+ his3200/his3200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-52/ura3-52 vhs2-Δ1∷TRP1/ vhs21∷TRP1 mlf3-Δ1∷kan/mlf3-Δ1∷kan
CCY1708-1Aaade2 his3-Δ200 leu2-3,112 lys2-801 ura3-52 spHIS5-pGAL1-3HA-SKG6
CCY1708-1A-1aade2 his3-Δ200 leu2-3,112 lys2-801 ura3-52 spHIS5-pGAL1-3HA-SKG6 MYO1-GFP∷LEU2
CCY1708-1A-2aade2 his3-Δ200 leu2-3,112 lys2-801 ura3-52 spHIS5-pGAL1-3HA-SKG6 HOF1-GFP∷LEU2
CCY1709-2Bahis3-Δ200 leu2-3,112 lys2-801 ura3-52 spHIS5-pGAL1-3HA-TOS2
CCY1710-3Caade2 his3-Δ200 leu2-3,112 lys2-801 ura3-52
CCY1710-3C-1aade2 his3-Δ200 leu2-3,112 lys2-801 ura3-52 MYO1-GFP∷LEU2
CCY1710-3C-2aade2 his3-Δ200 leu2-3,112 lys2-801 ura3-52 HOF1-GFP∷LEU2
CCY1720aade2/+ lys2-801/+ his3-Δ200/his3-Δ200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-52/ura3-52 vhs2-Δ1∷TRP1/vhs2-Δ1∷TRP1
CCY1721aade2/+ lys2-801/+ his3-Δ200/his3-Δ200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-52/ura3-52 mlf3-Δ1∷kan/mlf3-Δ1∷kan
CCY1726-18Cαlys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 gic1-Δ1∷LEU2 gic2-1∷HIS3
CCY1726-2Bαlys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 gic1-Δ1∷LEU2 gic2-1∷HIS3 vhs2-Δ1∷TRP1
CCY1726-4Dαlys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 gic1-Δ1∷LEU2 gic2-1∷HIS3 vhs2-Δ1∷TRP1 mlf3-Δ1∷kan
CCY1726-5Bαlys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52 gic1-Δ1∷LEU2 gic2-1∷HIS3 mlf3-Δ1∷kan
DBY1829αlys2-801 his3-Δ200 leu2-3,112 trp1-1 ura3-52
DBY1830aade2/+ lys2-801/+ his3-Δ200/his3-Δ200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-52/ura3-52
  • Strains DBY1829 and DBY1830 are from David Botstein's laboratory. Strain 1024-19C is from Chen et al. (1997). All other strains were constructed specifically for this study.