TABLE 4

Positions and sequences of substitution mutations in the subterminal region of Tol2 by MluI mutagenesis

Mutant nameaWild-type sequence (positionb)Mutant sequence
mTIRL1-6CAGAGGTGTAAAGTA (L1-15)ACGCGTTGTAAAGTA
mTIRR1-6CAGAGGTGTAAAAAG (R1-15)ACGCGTTGTAAAAAG
mL13-16TGTAAAGTACTTGAG (L7-21)TGTAAACGCGTTGAG
mL26-30GTAATTTTACTTGAT (L21-35)GTAATACGCGTTGAT
mL48-51ACTTAAGTATTATTT (L42-56)ACTTAACGCGTATTT
mL66-70GGATTTTTACTTTAC (L61-75)GGATTACGCGTTTAC
mL106-108CTTTTACTTTTACTT (L99-113)CTTTTACGCGTACTT
mL136-148AAAGTACTTTTTACT (L135-149)ATTTTACGCGTTTTT
mL169-181AGTCAAAAAGTACTT (L168-182)AAACACGCGTTAAAT
mR15-18TTTTGAGTACTTTTT (R24-10)TTTTGACGCGTTTTT
mR27-31ACTTGAGTAAAATTT (R37-23)ACTTGACGCGTATTT
mR48-51CCCTAAGTACTTGTA (R57-43)CCCTAACGCGTTGTA
mR63-67AATTGAGTAAAATTT (R73-59)AATTGACGCGTATTT
mR103-108TTTTACTCAAGTAAG (R113-99)TTTTAACGCGTTAAG
mR114-126GAGTACTTTTTTACT (R127-113)GTTTAACGCGTTTTT
mL88-92CAATTAAAAATCAAT (L83-97)CAATTACGCGTCAAT
mL158-162TACAATTTTATTTAC (L153-167)TACAAACGCGTTTAC
mR89-94GATTCTAGCCAGATA (R99-85)GATTCACGCGTGATA
  • The bases substituted in the mutants are underlined. TIR, terminal inverted repeat.

  • a L and R indicate mutations in the left end and the right end, respectively. Numbers indicate positions of the bases substituted in the mutants.

  • b The positions of the first and the last base of the sequence shown here are indicated in parentheses as base-pair distances from the left end (L) or the right end (R).