Positions and sequences of substitution mutations in the subterminal region of Tol2 by MluI mutagenesis
| Mutant namea | Wild-type sequence (positionb) | Mutant sequence |
|---|---|---|
| mTIRL1-6 | CAGAGGTGTAAAGTA (L1-15) | ACGCGTTGTAAAGTA |
| mTIRR1-6 | CAGAGGTGTAAAAAG (R1-15) | ACGCGTTGTAAAAAG |
| mL13-16 | TGTAAAGTACTTGAG (L7-21) | TGTAAACGCGTTGAG |
| mL26-30 | GTAATTTTACTTGAT (L21-35) | GTAATACGCGTTGAT |
| mL48-51 | ACTTAAGTATTATTT (L42-56) | ACTTAACGCGTATTT |
| mL66-70 | GGATTTTTACTTTAC (L61-75) | GGATTACGCGTTTAC |
| mL106-108 | CTTTTACTTTTACTT (L99-113) | CTTTTACGCGTACTT |
| mL136-148 | AAAGTACTTTTTACT (L135-149) | ATTTTACGCGTTTTT |
| mL169-181 | AGTCAAAAAGTACTT (L168-182) | AAACACGCGTTAAAT |
| mR15-18 | TTTTGAGTACTTTTT (R24-10) | TTTTGACGCGTTTTT |
| mR27-31 | ACTTGAGTAAAATTT (R37-23) | ACTTGACGCGTATTT |
| mR48-51 | CCCTAAGTACTTGTA (R57-43) | CCCTAACGCGTTGTA |
| mR63-67 | AATTGAGTAAAATTT (R73-59) | AATTGACGCGTATTT |
| mR103-108 | TTTTACTCAAGTAAG (R113-99) | TTTTAACGCGTTAAG |
| mR114-126 | GAGTACTTTTTTACT (R127-113) | GTTTAACGCGTTTTT |
| mL88-92 | CAATTAAAAATCAAT (L83-97) | CAATTACGCGTCAAT |
| mL158-162 | TACAATTTTATTTAC (L153-167) | TACAAACGCGTTTAC |
| mR89-94 | GATTCTAGCCAGATA (R99-85) | GATTCACGCGTGATA |
The bases substituted in the mutants are underlined. TIR, terminal inverted repeat.
↵a L and R indicate mutations in the left end and the right end, respectively. Numbers indicate positions of the bases substituted in the mutants.
↵b The positions of the first and the last base of the sequence shown here are indicated in parentheses as base-pair distances from the left end (L) or the right end (R).