TABLE 3

Plasmid “shuffle” assay with rad53 mutations in cdc7ts and cdc7ts mcm5-bob1 strains

cdc7ts mcm5-bob1ab
Plasmid for “shuffle”Mutant domain(s)cdc7tsa22°36°
VectorNA
RAD53+None+++
rad53 R70A N107AFHA1+++
rad53 NVS/AAAFHA2+++
rad53 H622AFHA2+++
rad53 K227A D339AKinase
rad53 R70A N107A NVS/AAAFHA1 FHA2+++
  • a Strains RSY1142 and RSY1143 rad53Δ sml1Δ cdc7 (pRAD53) were transformed with the indicated plasmids (Table 1). + or − indicates whether the initial pRAD53 plasmid could or could not be shuffled and replaced with the listed plasmid, respectively, at the permissive temperature of 22°.

  • b RSY1145 and RSY1195 rad53Δ sml1Δ cdc7 mcm5-bob1 (pRAD53) were transformed with the indicated plasmids (Table 1). + or − indicates whether the resultant rad53Δ sml1Δ cdc7 mcm5-bob1 strain with the shuffled plasmid listed could grow at the restrictive temperature of 36°. Growth at 36° indicates mcm5-bob1 suppression of cdc7ts.