TABLE 1

List of S. cerevisiae strains and recombinant plasmids used in this study

Strain or plasmidGenotypeSource/reference/alias
PDY037MATa ura3 his3 leu2 cdc7Δ1∷HIS3 mcm5-bob1-1 3HA-RAD53∷URA3Pessoa-Brandao and Sclafani (2004)
PDY201MATα cdc7-1 rad53-31 ade2 ade3 leu2 trp1 ura3 (pPD7 = YCp CDC7-ADE3)Dohrmann et al. (1999)
PDY253MATa cdc7-4 ade2 ura3This study
PDY258MATα rad53-11 pep4Δ∷URA3 ura3
PDY294MATα rad53Δ∷hisG-URA3-hisG his3 lys2 leu2 trp1 ura3 (pGAP-RNR1-2μ TRP)This study
PDY297MATα rad53Δ∷hisG his3 lys2 leu2 trp1 ura3 (pGAP-RNR1-2μ TRP)This study
PDY376MATa ura3 leu2 trp1 3HA-RAD53∷URA3Pessoa-Brandao and Sclafani (2004)
PDY420MATα cdc7-1 mcm5-bob1-2 rad53-31 ade2 ade3 leu2 trp1 ura3 (pPD7 = YCp CDC7-ADE3)Dohrmann et al. (1999)
PDY423MATa cdc7-4 rad53-11 pep4Δ∷URA3 mcm5-bob1-1 ura3This study
PDY425MATa his3 cdc7-4 mcm5-bob1-2 ura3 rad53-11 pep4Δ∷URA3Dohrmann et al. (1999)
PDY449MATα rad53Δ∷hisG URA3∷ lacZ vector his3 lys2 leu2 trp1 (pGAP-RNR1-2μ TRP)This study
PDY477MATα rad53Δ∷hisG URA3∷ARS1UAS-lacZ his3 lys2 leu2 trp1 ura3 (pGAP-RNR1-2μ TRP)This study
PDY478MATα rad53Δ∷hisG URA3∷1xF.ARS1UAS lacZ his3 lys2 leu2 trp1 ura3 (pGAP RNR12μ TRP)This study
PDY480MATa leu2 trp1 rad53Δ∷hisG URA3∷ARS.2xR (pGAP-RNR1-2μ TRP)This study
P101MATα leu2 his7 tyr1 cdc7-3 mcm5-bob1-1 lys2Jackson et al. (1993); Hardy et al. (1997)
P138MATα leu2 ura3 cdc7-1 mcm5-bob1-1 cyh2Jackson et al. (1993); Hardy et al. (1997)
P253MATa ura3 can1 trp1 his3 cdc7-1 bob1-1 cyh2Jackson et al. (1993); Hardy et al. (1997)
P142MATa leu2 ura3 cdc7-1 mcm5-bob1-1Jackson et al. (1993); Hardy et al. (1997)
1815-4CMATa ura3 mad2-1P. Megee
RSY299MATα his3 leu2 trp1 ura3Jackson et al. (1993); Hardy et al. (1997)
RSY 311MATa trp1 leu2 ura3 can1 his6 bar1Sclafani et al. (2002)
RSY465MATa leu2 ura3 trp1 his7 rad9Δ∷LEU27859-7-4 (T. Weinert)
RSY847MATa leu2 trp1 his3 ura3 cdc7-7 mcm5-bob1-2Pessoa-Brandao and Sclafani (2004)
RSY870MATa his3 trp1 mec1-1 sml1-1TWY304 (T. Weinert)
RSY1060MATa his3 leu2 trp1 ura3 sml1Δ∷hygro rad53Δ∷kanMX4JKT010 rad53 (J. Tyler)b
RSY1064MATa leu2 trp1 rad53Δ∷hisG URA3∷ARS.2xR (pGAP-RNR1-2μ LEU2)This study
RSY1104MATa mrc1Δ∷kanMX4 leu2 lys2 ura3 his3YCL060C (Open Biosystems)c
RSY1109MATα cdc7-1 trp1-289 leu2 ura3 his3 mcm5-bob1-1 mrc1Δ∷kanMX4This studyd
RSY1111MATα cdc7-1 trp1-289 leu2 ura3 his3 mrc1Δ∷kanMX4This studyd
RSY1113MATa chk1Δ∷URA3 his3 leu2 trp1 ura3YS152 Sanchez et al. (1996)b
RSY1128MATα leu2 ura3 cdc7-1 mcm5-bob1-1 cyh2P138 X RSY1060 (This study)a
MATa leu2 ura3 + + +
rad53Δ∷kanMX4 his3 trp1 sml1Δ∷hygro
+ + + +
RSY1139MATa leu2 ura3 his3 cdc7-7 tel1Δ∷kanMX4 trp1 mcm5-bob1-2This studya
RSY1141MATα mec1-1 sml1-1 cdc7-1 leu2 his3 ura1 ura3 cyh2This study
RSY1142MATa cdc7-1 leu2 ura3 rad53Δ∷kanMX4 his3 sml1Δ∷hygro (YCp URA3 RAD53)This studya
RSY1143MATa cdc7-1 leu2 ura3 rad53Δ∷kanMX4 his3 smlΔ1∷hygro (pARS CEN URA3 LEU2 RAD53)This studya
RSY1145MATα cdc7-1 mcm5-bob1-1 leu2 ura3 his3 rad53Δ∷kanMX4 sml1Δ∷hygro (pARS CEN URA3 RAD53)This studya
RSY1157MATa cdc7-1 leu2 ura3 rad53Δ∷kanMX4 his3 sml1Δ∷hygro hht2Δhhf2Δ∷HIS3 (pARS CEN URA3 RAD53)This studya
RSY1158MATα cdc7-1 mcm5-bob1-1 leu2 ura3 his3 rad53Δ∷kanMX4 sml1Δ∷hygro hht2Δhhf2Δ∷HIS3 (pARS CEN URA3 RAD53)This studya
RSY1161MATα cdc7-1 mcm5-bob1-1 arg4 ura3 can1 cyh2 his3 ade1 clb5∷ARG4 hht2Δhhf2Δ∷HIS3This study
RSY1162MATa ade2 his3 leu2 trp1 ura3 asf1Δ∷kanMX4JLY1017 (J. Tyler)b
RSY1163MATa ade2 his3 leu2 trp1 ura3 cac1Δ∷LEU2JLY1018 (J. Tyler)b
RSY1164MATa tel1Δ∷kanMX4 ura3 his3 leu2 met15YBL088C (Open Biosystems)c
RSY1177MATa cdc7-1 leu2 rad9Δ∷LEU2 mrc1Δ∷kanMX4This studyd
RSY1179MATα ura3 his3 lys2 leu2 dun1Δ∷kanMX4YDL101C (Open Biosystems)c
RSY1195MATα cdc7-1 mcm5-bob1-1 leu2 ura3 his3 rad53Δ∷kanMX4 sml1Δ∷hygro (YCp LEU2 RAD53)This studya
RSY1199MATα leu2 his3 cdc7-1 cac1Δ∷LEU2 ade2 ura3This studya
pPD1pRS316-CDC7 (URA3)Dohrmann et al. (1999)
pPD7pRS314-CDC7-ADE3 (TRP1)Dohrmann et al. (1999)
pPD60pRS316-RAD53+ (URA3)Dohrmann et al. (1999)
pRS423HIS3 2μ yeast shuttle vectorChristianson et al. (1992)
pGAP-RNR1pRNR1 from GAP pro (TRP1)T. Weinert
pPD348pRS423-GAP-RNR1 (HIS3)This study
pPD348pRS425-GAP-RNR1 (LEU2)This study
pRAS490mcm5-bob1-2 (URA3)Pessoa-Brandao and Sclafani (2004)
pDK243CEN4 (1x ARS) (LEU2)Hogan and Koshland (1992)
pDK368-7CEN4 (8x ARS) (LEU2)Hogan and Koshland (1992)
p305.2ARS305 CEN5 (URA3)Friedman et al. (1996)
pARS1ARS1 CEN5 (URA3)Friedman et al. (1996)
p12ARS1412 CEN5 (URA3)Friedman et al. (1996)
pLG-ARS1+ARS1-lacZ one-hybrid vector (URA3)Dowell et al. (1994)
pPD339pLGΔ178 (URA3, Δ2μ seq)Guarente and Mason (1983)
pPD342pLG-ARS1+ (URA3, Δ2μ seq)This study
pPD357pLG-1xF.ARS1 (URA3, Δ2μ seq)This study
pPD358pLG-1xR.ARS1 (URA3, Δ2μ seq)This study
pPD359pLG-2xR.ARS1 (URA3, Δ2μ seq)This study
pGAD-2FpGAL4AD (LEU2)Chien et al. (1991)
pUNI10Univector Plasmid SystemLiu et al. (1998)
pACT2-loxGAL4AD-fusion vectorLiu et al. (1998)
pPD206pUNI10-RAD53+This study
pPD233pACT2-lox-RAD53AD(LEU2)This study
pRS315LEU2 yeast shuttle vectorSikorski and Hieter (1989)
pPD361pRS315-RAD53 (LEU2)This study
pY2pGAL4DB (TRP1)Sadowski et al. (1992)
pPD94pGAL4DB-RAD53 (TRP1)Dohrmann et al. (1999)
pRS316-RAD53RAD53+ (URA3)Sun et al. (1996, 1998a,b)
pRS316-rad53kdrad53 K227A D339A (URA3)Sun et al. (1996)
pRS316-rad53-NVSrad53 NVS/AAA (655-657) (URA3)Sun et al. (1998a,b)
pRS316-rad53-H622Arad53 H622A (URA3)Sun et al. (1998a,b)
pRS315-RAD53RAD53+ (LEU2)Schwartz et al. (2003)
pRS315-rad53-fha1rad53 R70A N107A (LEU2)Schwartz et al. (2003)
pRS315-rad53-fha1,2rad53 R70A N107A NVS-AAA (655–657) (LEU2)Schwartz et al. (2003)
pJG46B42AD (TRP1) vectorDuncker et al. (2002)
pRAD53B42AD-RAD53+(TRP1)Duncker et al. (2002)
FHA1B42AD-rad53(I4-S165) (TRP1)Duncker et al. (2002)
KINB42AD-rad53(G177-N599) (TRP1)Duncker et al. (2002)
FHA2B42AD-rad53(M497-S821) (TRP1)Duncker et al. (2002)
fha1B42AD-rad53(I4-S165; R70A) (TRP1)Duncker et al. (2002)
fha2B42AD-rad53(M497-S821; R605A) (TRP1)Duncker et al. (2002)
pRM102Gal10p-HHT2 Gal1p-HHF2 (URA3)Mann and Grunstein (1992)
  • All yeast strains are congenic with A364a except if indicated otherwise:

  • a W303/A364a hybrid.

  • b W303.

  • c S288C.

  • d S288C/A364a hybrid.