TABLE 1

SNPs identified and tested in this study

SNPPrimer APrimer BNo. of SNPs in ampliconB6/BALB alleleCAST allele
SNP-8465′-GGCTAAGCCATCACTTATCC-3′5′-AAGATTCCTAACTGTTCTGGG-3′3 (all in dbSNP)GCTTTC
SNP-8285′-CCATGGAGATGATGACAAGC-3′5′-GGCCTACAAAGCGACTTCC-3′1 novelAG
LeeSNPG-Ia5′-GCTTGGTTCGTCTATCTTGTGGG-3′5′-CCAGAGTCTGATGTAACGGAGG-3′1Not digested by ScrFIDigested by ScrFI
SNP-8435′-TGTTTCCATGCCTCAGAAGC-3′5′-GAACCACACTGCTTAACTAGC-3′3 novel 2 in dbSNPTACCAACTAG
  • SNPs were genotyped by PCR amplification of genomic DNA using the primers indicated followed by sequencing.

  • a This assay was published in Stavropoulos et al. (2001). To genotype this SNP, genomic DNA was PCR amplified with the primers indicated and digested with ScrFI, and products were separated by gel electrophoresis.