Numbers of recombinants and rates of unequal recombination

CrossaFemale genotypecNo. isolatedNo. testeddNo. confirmedNo. correctedePopulation sizefRate (× 10−4)g
A. At A1-b
2AbA1-b Sh2/a1∷rdt Sh2432342223282459,4006.1 ± 0.4
205159119153190,8008.0 ± 0.6
3AA1-b Sh2/ax-12131181527454,8000.59 ± 0.14
4AA1-b Sh2/A1-b Sh21248989124165,7007.5 ± 0.7
B. At A1-b(P415)
2BA1-b(P415) Sh2/a1∷rdt Sh2450343212278418,7006.6 ± 0.4
3BA1-b(P415) Sh2/ax-1466329194275338,9008.1 ± 0.5
4BA1-b(P415) Sh2/A1-b(P415) Sh22421131539,1003.8 ± 1.0
  • a Cross from which pale round recombinants were isolated.

  • b Crosses 2A–4A were conducted in two separate isolation plots. For cross 2A, unlike in crosses 3A and 4A, the rates of unequal recombination differed significantly between plots and both rates are therefore presented.

  • c The genotype from which pale round recombinants were isolated.

  • d Putative pale recombinants were tested by genetic crosses and PCR analyses with the primers in Table 1.

  • e No. corrected = no. isolated × (no. confirmed/no. tested).

  • f Population sizes are based on the number of gametes containing the A1-b Sh2 chromosome.

  • g Standard errors were calculated using the formula (pq/n)1/2.