Analysis of genes expressed in only one inbred based on microarray data

PCR of genomic DNAPCR of cDNAa
B73 GSS matchB73 amplicon onlyMo17 amplicon onlyAmplicons in B73 and Mo17No ampliconsPresent–absent expression validatedExpression detected in B73 and Mo17Expression not detected in B73 or Mo17
B73-expressed genes83/947381348107
Mo17-expressed genes33/431229112140
  • ↵a The PCR validation using a cDNA template was performed using 90 primer sets that had genomic amplification products in both B73 and Mo17.